Photoautotrophic culture with CO2 enrichment for improving micropropagation of Coffea arabusta using somatic embryos

A. Uno, K. Ohyama, T. Kozai, Chieri Kubota

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Coffea arabusta somatic embryos were cultured photoautotrophically (without sugar in the medium) at different CO2 concentrations, and conversion of the somatic embryos was compared to that cultured conventionally (with sugar in the medium). Torpedo-shaped and cotyledonary embryos were cultured photoautotrophically in a medium with a vermiculite-based supporting material in vessels with 0.8 h-1 air exchanges under 100 μmol m-2 s-1 photosynthetic photon flux (PPF) at one of three CO2 concentrations [400 (ambient), 1500 and 5000 μmol mol-1], or conventionally in an agar-gelled medium with 20 g/L sucrose in vessels with 0.1 h-1 air exchanges under 30 μmol m-2 s-1 PPF at 400 μmol mol-1 CO2 for 61 days. When cultured photoautotrophically at 1500 or 5000 μmol mol-1 CO2, percent conversion of cotyledonary embryos to plantlets attained 55-60%, a significantly higher percentage than that when cultured conventionally (17%). The conversion at 400 μmol mol-1 CO2 under photoautotrophic conditions was not significantly different from that under the conventional conditions. The percent conversion of torpedo-shaped to cotyledonary embryos was 90-100% when cultured conventionally or photoautotrophically at 5000 μmol mol-1 CO2, while it was 20-60% when cultured photoautotrophically at the lower CO2 concentrations, where 15-50% torpedo-shaped embryos died. Cotyledonary embryos exhibited greater fresh and dry mass at 5000 μmol mol-1 CO2 under photoautotrophic conditions than under conventional conditions. When cultured photoautotrophically at 5000 μmol mol-1 CO2, torpedo-shaped embryos attained fresh and dry mass comparable to those under conventional conditions. Photoautotrophic culture was applicable to Coffea arabusta for improving micropropagation using somatic embryos at torpedo and cotyledonary stages provided that the CO2 concentration was properly controlled.

Original languageEnglish (US)
Title of host publicationActa Horticulturae
Pages271-277
Number of pages7
Volume625
StatePublished - Sep 30 2003

Publication series

NameActa Horticulturae
Volume625
ISSN (Print)05677572

Fingerprint

Coffea
micropropagation
somatic embryos
carbon dioxide
Torpedo
embryo (plant)
sugars
air
vermiculite
plantlets
agar
sucrose

Keywords

  • CO concentration
  • Controlled environment
  • Conversion
  • Cotyledonary embryo
  • In vitro culture
  • Photosynthesis
  • Somatic embryogenesis
  • Torpedo-shaped embryo

ASJC Scopus subject areas

  • Horticulture

Cite this

Uno, A., Ohyama, K., Kozai, T., & Kubota, C. (2003). Photoautotrophic culture with CO2 enrichment for improving micropropagation of Coffea arabusta using somatic embryos. In Acta Horticulturae (Vol. 625, pp. 271-277). (Acta Horticulturae; Vol. 625).

Photoautotrophic culture with CO2 enrichment for improving micropropagation of Coffea arabusta using somatic embryos. / Uno, A.; Ohyama, K.; Kozai, T.; Kubota, Chieri.

Acta Horticulturae. Vol. 625 2003. p. 271-277 (Acta Horticulturae; Vol. 625).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Uno, A, Ohyama, K, Kozai, T & Kubota, C 2003, Photoautotrophic culture with CO2 enrichment for improving micropropagation of Coffea arabusta using somatic embryos. in Acta Horticulturae. vol. 625, Acta Horticulturae, vol. 625, pp. 271-277.
Uno A, Ohyama K, Kozai T, Kubota C. Photoautotrophic culture with CO2 enrichment for improving micropropagation of Coffea arabusta using somatic embryos. In Acta Horticulturae. Vol. 625. 2003. p. 271-277. (Acta Horticulturae).
Uno, A. ; Ohyama, K. ; Kozai, T. ; Kubota, Chieri. / Photoautotrophic culture with CO2 enrichment for improving micropropagation of Coffea arabusta using somatic embryos. Acta Horticulturae. Vol. 625 2003. pp. 271-277 (Acta Horticulturae).
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abstract = "Coffea arabusta somatic embryos were cultured photoautotrophically (without sugar in the medium) at different CO2 concentrations, and conversion of the somatic embryos was compared to that cultured conventionally (with sugar in the medium). Torpedo-shaped and cotyledonary embryos were cultured photoautotrophically in a medium with a vermiculite-based supporting material in vessels with 0.8 h-1 air exchanges under 100 μmol m-2 s-1 photosynthetic photon flux (PPF) at one of three CO2 concentrations [400 (ambient), 1500 and 5000 μmol mol-1], or conventionally in an agar-gelled medium with 20 g/L sucrose in vessels with 0.1 h-1 air exchanges under 30 μmol m-2 s-1 PPF at 400 μmol mol-1 CO2 for 61 days. When cultured photoautotrophically at 1500 or 5000 μmol mol-1 CO2, percent conversion of cotyledonary embryos to plantlets attained 55-60{\%}, a significantly higher percentage than that when cultured conventionally (17{\%}). The conversion at 400 μmol mol-1 CO2 under photoautotrophic conditions was not significantly different from that under the conventional conditions. The percent conversion of torpedo-shaped to cotyledonary embryos was 90-100{\%} when cultured conventionally or photoautotrophically at 5000 μmol mol-1 CO2, while it was 20-60{\%} when cultured photoautotrophically at the lower CO2 concentrations, where 15-50{\%} torpedo-shaped embryos died. Cotyledonary embryos exhibited greater fresh and dry mass at 5000 μmol mol-1 CO2 under photoautotrophic conditions than under conventional conditions. When cultured photoautotrophically at 5000 μmol mol-1 CO2, torpedo-shaped embryos attained fresh and dry mass comparable to those under conventional conditions. Photoautotrophic culture was applicable to Coffea arabusta for improving micropropagation using somatic embryos at torpedo and cotyledonary stages provided that the CO2 concentration was properly controlled.",
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N2 - Coffea arabusta somatic embryos were cultured photoautotrophically (without sugar in the medium) at different CO2 concentrations, and conversion of the somatic embryos was compared to that cultured conventionally (with sugar in the medium). Torpedo-shaped and cotyledonary embryos were cultured photoautotrophically in a medium with a vermiculite-based supporting material in vessels with 0.8 h-1 air exchanges under 100 μmol m-2 s-1 photosynthetic photon flux (PPF) at one of three CO2 concentrations [400 (ambient), 1500 and 5000 μmol mol-1], or conventionally in an agar-gelled medium with 20 g/L sucrose in vessels with 0.1 h-1 air exchanges under 30 μmol m-2 s-1 PPF at 400 μmol mol-1 CO2 for 61 days. When cultured photoautotrophically at 1500 or 5000 μmol mol-1 CO2, percent conversion of cotyledonary embryos to plantlets attained 55-60%, a significantly higher percentage than that when cultured conventionally (17%). The conversion at 400 μmol mol-1 CO2 under photoautotrophic conditions was not significantly different from that under the conventional conditions. The percent conversion of torpedo-shaped to cotyledonary embryos was 90-100% when cultured conventionally or photoautotrophically at 5000 μmol mol-1 CO2, while it was 20-60% when cultured photoautotrophically at the lower CO2 concentrations, where 15-50% torpedo-shaped embryos died. Cotyledonary embryos exhibited greater fresh and dry mass at 5000 μmol mol-1 CO2 under photoautotrophic conditions than under conventional conditions. When cultured photoautotrophically at 5000 μmol mol-1 CO2, torpedo-shaped embryos attained fresh and dry mass comparable to those under conventional conditions. Photoautotrophic culture was applicable to Coffea arabusta for improving micropropagation using somatic embryos at torpedo and cotyledonary stages provided that the CO2 concentration was properly controlled.

AB - Coffea arabusta somatic embryos were cultured photoautotrophically (without sugar in the medium) at different CO2 concentrations, and conversion of the somatic embryos was compared to that cultured conventionally (with sugar in the medium). Torpedo-shaped and cotyledonary embryos were cultured photoautotrophically in a medium with a vermiculite-based supporting material in vessels with 0.8 h-1 air exchanges under 100 μmol m-2 s-1 photosynthetic photon flux (PPF) at one of three CO2 concentrations [400 (ambient), 1500 and 5000 μmol mol-1], or conventionally in an agar-gelled medium with 20 g/L sucrose in vessels with 0.1 h-1 air exchanges under 30 μmol m-2 s-1 PPF at 400 μmol mol-1 CO2 for 61 days. When cultured photoautotrophically at 1500 or 5000 μmol mol-1 CO2, percent conversion of cotyledonary embryos to plantlets attained 55-60%, a significantly higher percentage than that when cultured conventionally (17%). The conversion at 400 μmol mol-1 CO2 under photoautotrophic conditions was not significantly different from that under the conventional conditions. The percent conversion of torpedo-shaped to cotyledonary embryos was 90-100% when cultured conventionally or photoautotrophically at 5000 μmol mol-1 CO2, while it was 20-60% when cultured photoautotrophically at the lower CO2 concentrations, where 15-50% torpedo-shaped embryos died. Cotyledonary embryos exhibited greater fresh and dry mass at 5000 μmol mol-1 CO2 under photoautotrophic conditions than under conventional conditions. When cultured photoautotrophically at 5000 μmol mol-1 CO2, torpedo-shaped embryos attained fresh and dry mass comparable to those under conventional conditions. Photoautotrophic culture was applicable to Coffea arabusta for improving micropropagation using somatic embryos at torpedo and cotyledonary stages provided that the CO2 concentration was properly controlled.

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