Abstract
Photoautotrophic micropropagation (no sugar in the medium under a controlled environment) has many advantages over conventional photomixotrophic micropropagation (using sugar-containing medium), such as promoted growth and development of plantlets in vitro and enhanced percent survival ex vitro with many different plant species. Rhododendron is an important ornamental woody species, which often shows low multiplication rates and low percentages of rooting in vitro and survival ex vitro in conventional photomixotrophic micropropagation, and which could possibly benefit from photoautotrophic culture. In the present study, photoautotrophic culture was investigated during the multiplication and preparation stages of Rhododendron micropropagation. Based on the results, suitable conditions for obtaining a large number of Rhododendron plantlets with enhanced growth included the absence of plant growth regulators in the medium, a relatively high photosynthetic photon flux, a high CO2 concentration, two-leafed nodal cuttings as expiants and full-strength medium. Normal roots were obtained in the absence of plant growth regulators, although the percentages of rooted plantlets were low. Further research is needed to enhance rooting in photoautotrophic micropropagation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 385-390 |
| Number of pages | 6 |
| Journal | Progress in Biotechnology |
| Volume | 18 |
| Issue number | C |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- 2iP
- CO concentration
- controlled environment
- expiant type
- IAA
- IBA
- medium strength
- multiplication ratio
- PPF
- sugar
ASJC Scopus subject areas
- Biotechnology
Cite this
Photoautotrophic micropropagation of Rhododendron. / Valero-Aracama, Carmen; Zobayed, Sayed M A; Roy, Shyamal K.; Kubota, Chieri; Kozai, Toyoki.
In: Progress in Biotechnology, Vol. 18, No. C, 2001, p. 385-390.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photoautotrophic micropropagation of Rhododendron
AU - Valero-Aracama, Carmen
AU - Zobayed, Sayed M A
AU - Roy, Shyamal K.
AU - Kubota, Chieri
AU - Kozai, Toyoki
PY - 2001
Y1 - 2001
N2 - Photoautotrophic micropropagation (no sugar in the medium under a controlled environment) has many advantages over conventional photomixotrophic micropropagation (using sugar-containing medium), such as promoted growth and development of plantlets in vitro and enhanced percent survival ex vitro with many different plant species. Rhododendron is an important ornamental woody species, which often shows low multiplication rates and low percentages of rooting in vitro and survival ex vitro in conventional photomixotrophic micropropagation, and which could possibly benefit from photoautotrophic culture. In the present study, photoautotrophic culture was investigated during the multiplication and preparation stages of Rhododendron micropropagation. Based on the results, suitable conditions for obtaining a large number of Rhododendron plantlets with enhanced growth included the absence of plant growth regulators in the medium, a relatively high photosynthetic photon flux, a high CO2 concentration, two-leafed nodal cuttings as expiants and full-strength medium. Normal roots were obtained in the absence of plant growth regulators, although the percentages of rooted plantlets were low. Further research is needed to enhance rooting in photoautotrophic micropropagation.
AB - Photoautotrophic micropropagation (no sugar in the medium under a controlled environment) has many advantages over conventional photomixotrophic micropropagation (using sugar-containing medium), such as promoted growth and development of plantlets in vitro and enhanced percent survival ex vitro with many different plant species. Rhododendron is an important ornamental woody species, which often shows low multiplication rates and low percentages of rooting in vitro and survival ex vitro in conventional photomixotrophic micropropagation, and which could possibly benefit from photoautotrophic culture. In the present study, photoautotrophic culture was investigated during the multiplication and preparation stages of Rhododendron micropropagation. Based on the results, suitable conditions for obtaining a large number of Rhododendron plantlets with enhanced growth included the absence of plant growth regulators in the medium, a relatively high photosynthetic photon flux, a high CO2 concentration, two-leafed nodal cuttings as expiants and full-strength medium. Normal roots were obtained in the absence of plant growth regulators, although the percentages of rooted plantlets were low. Further research is needed to enhance rooting in photoautotrophic micropropagation.
KW - 2iP
KW - CO concentration
KW - controlled environment
KW - expiant type
KW - IAA
KW - IBA
KW - medium strength
KW - multiplication ratio
KW - PPF
KW - sugar
UR - http://www.scopus.com/inward/record.url?scp=77957042511&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957042511&partnerID=8YFLogxK
U2 - 10.1016/S0921-0423(01)80095-2
DO - 10.1016/S0921-0423(01)80095-2
M3 - Article
VL - 18
SP - 385
EP - 390
JO - Progress in Biotechnology
JF - Progress in Biotechnology
SN - 0921-0423
IS - C
ER -