Photoelectron spectroscopy of dithiolatodiironhexacarbonyl models for the active site of [Fe-Fe] hydrogenases

Insight into the reorganization energy of the "rotated" structure in the enzyme

Benjamin J. Petro, Aaron K. Vannucci, L. Tori Lockett, Charles Mebi, Rudresha Kottani, Nadine E. Gruhn, Gary S. Nichol, Paul A J Goodyer, Dennis H. Evans, Richard S Glass, Dennis L Lichtenberger

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Synthetic analogs, μ-(RS)2Fe2(CO)6, of the active site of [Fe-Fe] hydrogenases do not have the semi-bridged CO and "rotated" structure found in the enzyme. However, recent studies have shown that cations of dithiolatodiiron complexes adopt this rotated structure. This paper reports the use of photoelectron spectroscopy in combination with density functional theory calculations to show that two previously reported complexes: μ-(1,2-benzenedithiolato)Fe2(CO)6 and μ-(1,3-propanedithiolato)Fe2(CO)6 and two new complexes: μ-(2,3-pyridinodithiolato)Fe2(CO)6 and μ-(norbornane-2-exo,3-exo-dithiolato)Fe2(CO)6 favor the "rotated" structure in their corresponding cations. Furthermore, these methods provide a measure of the reorganization energy between the "rotated" and "unrotated" structures in the gas phase. The results provide insight on the entatic state of the dithiolatodiiron site in the enzyme, in which the protein controls the structure of the active site. This structure influences the redox energy and reorganization energy enabling fast electron transfer.

Original languageEnglish (US)
Pages (from-to)281-288
Number of pages8
JournalJournal of Molecular Structure
Volume890
Issue number1-3
DOIs
StatePublished - Nov 12 2008

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Carbon Monoxide
Photoelectron spectroscopy
Enzymes
Cations
Norbornanes
Density functional theory
iron hydrogenase
Gases
Electrons
Proteins

Keywords

  • Density functional theory
  • Hydrogenase
  • Metal-carbonyl clusters
  • Photoelectron spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Analytical Chemistry
  • Inorganic Chemistry
  • Organic Chemistry

Cite this

Photoelectron spectroscopy of dithiolatodiironhexacarbonyl models for the active site of [Fe-Fe] hydrogenases : Insight into the reorganization energy of the "rotated" structure in the enzyme. / Petro, Benjamin J.; Vannucci, Aaron K.; Lockett, L. Tori; Mebi, Charles; Kottani, Rudresha; Gruhn, Nadine E.; Nichol, Gary S.; Goodyer, Paul A J; Evans, Dennis H.; Glass, Richard S; Lichtenberger, Dennis L.

In: Journal of Molecular Structure, Vol. 890, No. 1-3, 12.11.2008, p. 281-288.

Research output: Contribution to journalArticle

Petro, Benjamin J. ; Vannucci, Aaron K. ; Lockett, L. Tori ; Mebi, Charles ; Kottani, Rudresha ; Gruhn, Nadine E. ; Nichol, Gary S. ; Goodyer, Paul A J ; Evans, Dennis H. ; Glass, Richard S ; Lichtenberger, Dennis L. / Photoelectron spectroscopy of dithiolatodiironhexacarbonyl models for the active site of [Fe-Fe] hydrogenases : Insight into the reorganization energy of the "rotated" structure in the enzyme. In: Journal of Molecular Structure. 2008 ; Vol. 890, No. 1-3. pp. 281-288.
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AU - Nichol, Gary S.

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