Photoinduced destabilization of liposomes

Henry Lamparski, Ulrich Liman, Judith A. Barry, David A. Frankel, V. Ramaswami, Michael F Brown, David F. O'Brien

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The stability of two-component liposomes composed of the polymerizable 1,2-bis-[10-(2′,4′-hexadienoyloxy)decanoyl]-sn-glycero-3- phosphatidylcholine (SorbPC) and either a phosphatidylethanolamine (PE) or a phosphatidylcholine (PC) were examined via fluorescence leakage assays. Ultraviolet light exposure of SorbPC-containing liposomes forms poly-SorbPC, which phase separates from the remaining monomeric lipids. If the nonpolymerizable lipids are PE's, then the photoinduced polymerization destabilizes the liposome with loss of aqueous contents. The permeability of the control dioleoylPC/SorbPC membranes was not affected by photopolymerization of SorbPC. The photodestabilization of dioleoylPE/SorbPC (3:1) liposomes required the presence of oligolamellar liposomes. NMR spectroscopy of extended bilayers of dioleoylPE/SorbPC (3:1) showed that the photopolymerization lowers the temperature for the appearance of 31P NMR signals due to the formation of isotropically symmetric lipid structures. These observations suggest the following model for the photoinduced destabilization of liposomes composed of PE/SorbPC: photopolymerization induced phase separation with the formation of enriched domains of PE, which allows the close approach of apposed regions of enriched PE lamellae and permits the formation of an isotropically symmetric structure between the lamellae. The formation of such an interlamellar attachment (ILA) between the lamellae of an oligolamellar liposome provides a permeability pathway for the light-stimulated leakage of entrapped water-soluble reagents.

Original languageEnglish (US)
Pages (from-to)685-694
Number of pages10
JournalBiochemistry
Volume31
Issue number3
StatePublished - 1992

Fingerprint

Liposomes
Photopolymerization
Phosphatidylcholines
Lipids
Permeability
1,2-bis(10(2',4'-hexadienoyloxy)decanoyl)-sn-glycero-3-phosphocholine
Ultraviolet Rays
Polymerization
Phase separation
Nuclear magnetic resonance spectroscopy
Assays
Magnetic Resonance Spectroscopy
Fluorescence
Nuclear magnetic resonance
Membranes
Light
Temperature
phosphatidylethanolamine
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lamparski, H., Liman, U., Barry, J. A., Frankel, D. A., Ramaswami, V., Brown, M. F., & O'Brien, D. F. (1992). Photoinduced destabilization of liposomes. Biochemistry, 31(3), 685-694.

Photoinduced destabilization of liposomes. / Lamparski, Henry; Liman, Ulrich; Barry, Judith A.; Frankel, David A.; Ramaswami, V.; Brown, Michael F; O'Brien, David F.

In: Biochemistry, Vol. 31, No. 3, 1992, p. 685-694.

Research output: Contribution to journalArticle

Lamparski, H, Liman, U, Barry, JA, Frankel, DA, Ramaswami, V, Brown, MF & O'Brien, DF 1992, 'Photoinduced destabilization of liposomes', Biochemistry, vol. 31, no. 3, pp. 685-694.
Lamparski H, Liman U, Barry JA, Frankel DA, Ramaswami V, Brown MF et al. Photoinduced destabilization of liposomes. Biochemistry. 1992;31(3):685-694.
Lamparski, Henry ; Liman, Ulrich ; Barry, Judith A. ; Frankel, David A. ; Ramaswami, V. ; Brown, Michael F ; O'Brien, David F. / Photoinduced destabilization of liposomes. In: Biochemistry. 1992 ; Vol. 31, No. 3. pp. 685-694.
@article{7fca6f56bc494da4b8f055a9ea7bb6fb,
title = "Photoinduced destabilization of liposomes",
abstract = "The stability of two-component liposomes composed of the polymerizable 1,2-bis-[10-(2′,4′-hexadienoyloxy)decanoyl]-sn-glycero-3- phosphatidylcholine (SorbPC) and either a phosphatidylethanolamine (PE) or a phosphatidylcholine (PC) were examined via fluorescence leakage assays. Ultraviolet light exposure of SorbPC-containing liposomes forms poly-SorbPC, which phase separates from the remaining monomeric lipids. If the nonpolymerizable lipids are PE's, then the photoinduced polymerization destabilizes the liposome with loss of aqueous contents. The permeability of the control dioleoylPC/SorbPC membranes was not affected by photopolymerization of SorbPC. The photodestabilization of dioleoylPE/SorbPC (3:1) liposomes required the presence of oligolamellar liposomes. NMR spectroscopy of extended bilayers of dioleoylPE/SorbPC (3:1) showed that the photopolymerization lowers the temperature for the appearance of 31P NMR signals due to the formation of isotropically symmetric lipid structures. These observations suggest the following model for the photoinduced destabilization of liposomes composed of PE/SorbPC: photopolymerization induced phase separation with the formation of enriched domains of PE, which allows the close approach of apposed regions of enriched PE lamellae and permits the formation of an isotropically symmetric structure between the lamellae. The formation of such an interlamellar attachment (ILA) between the lamellae of an oligolamellar liposome provides a permeability pathway for the light-stimulated leakage of entrapped water-soluble reagents.",
author = "Henry Lamparski and Ulrich Liman and Barry, {Judith A.} and Frankel, {David A.} and V. Ramaswami and Brown, {Michael F} and O'Brien, {David F.}",
year = "1992",
language = "English (US)",
volume = "31",
pages = "685--694",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Photoinduced destabilization of liposomes

AU - Lamparski, Henry

AU - Liman, Ulrich

AU - Barry, Judith A.

AU - Frankel, David A.

AU - Ramaswami, V.

AU - Brown, Michael F

AU - O'Brien, David F.

PY - 1992

Y1 - 1992

N2 - The stability of two-component liposomes composed of the polymerizable 1,2-bis-[10-(2′,4′-hexadienoyloxy)decanoyl]-sn-glycero-3- phosphatidylcholine (SorbPC) and either a phosphatidylethanolamine (PE) or a phosphatidylcholine (PC) were examined via fluorescence leakage assays. Ultraviolet light exposure of SorbPC-containing liposomes forms poly-SorbPC, which phase separates from the remaining monomeric lipids. If the nonpolymerizable lipids are PE's, then the photoinduced polymerization destabilizes the liposome with loss of aqueous contents. The permeability of the control dioleoylPC/SorbPC membranes was not affected by photopolymerization of SorbPC. The photodestabilization of dioleoylPE/SorbPC (3:1) liposomes required the presence of oligolamellar liposomes. NMR spectroscopy of extended bilayers of dioleoylPE/SorbPC (3:1) showed that the photopolymerization lowers the temperature for the appearance of 31P NMR signals due to the formation of isotropically symmetric lipid structures. These observations suggest the following model for the photoinduced destabilization of liposomes composed of PE/SorbPC: photopolymerization induced phase separation with the formation of enriched domains of PE, which allows the close approach of apposed regions of enriched PE lamellae and permits the formation of an isotropically symmetric structure between the lamellae. The formation of such an interlamellar attachment (ILA) between the lamellae of an oligolamellar liposome provides a permeability pathway for the light-stimulated leakage of entrapped water-soluble reagents.

AB - The stability of two-component liposomes composed of the polymerizable 1,2-bis-[10-(2′,4′-hexadienoyloxy)decanoyl]-sn-glycero-3- phosphatidylcholine (SorbPC) and either a phosphatidylethanolamine (PE) or a phosphatidylcholine (PC) were examined via fluorescence leakage assays. Ultraviolet light exposure of SorbPC-containing liposomes forms poly-SorbPC, which phase separates from the remaining monomeric lipids. If the nonpolymerizable lipids are PE's, then the photoinduced polymerization destabilizes the liposome with loss of aqueous contents. The permeability of the control dioleoylPC/SorbPC membranes was not affected by photopolymerization of SorbPC. The photodestabilization of dioleoylPE/SorbPC (3:1) liposomes required the presence of oligolamellar liposomes. NMR spectroscopy of extended bilayers of dioleoylPE/SorbPC (3:1) showed that the photopolymerization lowers the temperature for the appearance of 31P NMR signals due to the formation of isotropically symmetric lipid structures. These observations suggest the following model for the photoinduced destabilization of liposomes composed of PE/SorbPC: photopolymerization induced phase separation with the formation of enriched domains of PE, which allows the close approach of apposed regions of enriched PE lamellae and permits the formation of an isotropically symmetric structure between the lamellae. The formation of such an interlamellar attachment (ILA) between the lamellae of an oligolamellar liposome provides a permeability pathway for the light-stimulated leakage of entrapped water-soluble reagents.

UR - http://www.scopus.com/inward/record.url?scp=0026592702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026592702&partnerID=8YFLogxK

M3 - Article

C2 - 1731924

AN - SCOPUS:0026592702

VL - 31

SP - 685

EP - 694

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 3

ER -