Photosensitization of DNA damage by glycated proteins

Georg T Wondrak, Elaine L. Jacobson, Myron K. Jacobson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Photosensitized DNA damage in skin is thought to be an important mechanism of UV phototoxicity. Here we demonstrate that proteins modified by advanced glycation endproducts (AGE-proteins) are photosensitizers of DNA damage and show that multiple mechanisms are involved in AGE-sensitization. AGE-chromophores accumulate on long-lived skin proteins such as collagen and elastin as a consequence of glycation, the spontaneous amino-carbonyl reaction of protein-bound lysine and arginine residues with reactive carbonyl species. AGE-proteins accumulate in both the nucleus and the cytoplasm of mammalian cells. To test the hypothesis that protein-bound AGEs in close proximity to DNA are potent UV-photosensitizers, a simple plasmid DNA cleavage assay was established. Irradiation of supercoiled ΦX 174 DNA with solar simulated light in the presence of AGE-modified bovine serum albumin or AGE-modified RNAse A induced DNA single strand breaks. The sensitization potency of the glycated protein correlated with increased AGE-modification and the unmodified protein displayed no photosensitizing activity. AGE-sensitized formation of reactive oxygen species was not fully responsible for the observed DNA damage and other mechanisms such as direct electron transfer interaction between photoexcited AGE and DNA are likely to be involved. Glycated proteins in skin may equally function as potent photosensitizers of DNA damage with implications for photoaging and photocarcinogenesis.

Original languageEnglish (US)
Pages (from-to)355-363
Number of pages9
JournalPhotochemical and Photobiological Sciences
Volume1
Issue number5
DOIs
StatePublished - May 1 2002

Fingerprint

Photosensitivity Disorders
DNA Damage
deoxyribonucleic acid
damage
proteins
DNA
Proteins
Photosensitizing Agents
Skin
elastin
Phototoxic Dermatitis
Single-Stranded DNA Breaks
DNA Cleavage
plasmids
cytoplasm
Elastin
lysine
collagens
Chromophores
Bovine Serum Albumin

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Photosensitization of DNA damage by glycated proteins. / Wondrak, Georg T; Jacobson, Elaine L.; Jacobson, Myron K.

In: Photochemical and Photobiological Sciences, Vol. 1, No. 5, 01.05.2002, p. 355-363.

Research output: Contribution to journalArticle

Wondrak, Georg T ; Jacobson, Elaine L. ; Jacobson, Myron K. / Photosensitization of DNA damage by glycated proteins. In: Photochemical and Photobiological Sciences. 2002 ; Vol. 1, No. 5. pp. 355-363.
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