Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis

Allan Heres, Donald V Lightner

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalJournal of Invertebrate Pathology
Volume103
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Spiroplasma
phylogenetics
DNA
bacterium
shrimp
phylogeny
bacteria
nucleotide sequences
gene
crustacean
emerging diseases
Litopenaeus vannamei
substitution
pond
intergenic DNA
farm
Colombia
hemolymph
genetic distance
salinity

Keywords

  • Multilocus
  • Penaeus vannamei
  • Phylogenetic analysis
  • Spiroplasma penaei

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis. / Heres, Allan; Lightner, Donald V.

In: Journal of Invertebrate Pathology, Vol. 103, No. 1, 01.2010, p. 30-35.

Research output: Contribution to journalArticle

@article{43c4839631b6446ab5e53477aa61ecfe,
title = "Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis",
abstract = "A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.",
keywords = "Multilocus, Penaeus vannamei, Phylogenetic analysis, Spiroplasma penaei",
author = "Allan Heres and Lightner, {Donald V}",
year = "2010",
month = "1",
doi = "10.1016/j.jip.2009.10.004",
language = "English (US)",
volume = "103",
pages = "30--35",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Phylogenetic analysis of the pathogenic bacteria Spiroplasma penaei based on multilocus sequence analysis

AU - Heres, Allan

AU - Lightner, Donald V

PY - 2010/1

Y1 - 2010/1

N2 - A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.

AB - A pathogenic Spiroplasma penaei strain was isolated from the hemolymph of moribund Pacific white shrimp, Penaeus vannamei. The shrimp sample originated from a shrimp farm near Cartagena, Colombia, that was suffering from high mortalities in ponds with very low salinity and high temperatures. This new emerging disease in a marine crustacean in the Americas is described as a systemic infection. The multilocus phylogenetic analysis suggests that S. penaei strain has a terrestrial origin. Evolutionary relationship trees, based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S rDNA and 23S-5S rDNA intergenic spacer region, were reconstructed using the distance-based Neighboring-Joining (NJ) method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences investigated in this study positioned S. penaei in the Citri-Poulsonii clade and corroborate the observations by other investigators using the 16S rDNA gene. Pairwise genetic distance calculation between sequences of spiroplasmas showed S. penaei to be closely related to Spiroplasma insolitum and distantly related to Spiroplasma sp. SHRIMP from China.

KW - Multilocus

KW - Penaeus vannamei

KW - Phylogenetic analysis

KW - Spiroplasma penaei

UR - http://www.scopus.com/inward/record.url?scp=72049121486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72049121486&partnerID=8YFLogxK

U2 - 10.1016/j.jip.2009.10.004

DO - 10.1016/j.jip.2009.10.004

M3 - Article

C2 - 19836398

AN - SCOPUS:72049121486

VL - 103

SP - 30

EP - 35

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

IS - 1

ER -