Physical characterization of the "immunosignaturing effect"

Phillip Stafford, Rebecca Halperin, Joseph Bart Legutki, Dewey Mitchell Magee, John N Galgiani, Stephen Albert Johnston

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Identifying new, effective biomarkers for diseases is proving to be a challenging problem. We have proposed that antibodies may offer a solution to this problem. The physical features and abundance of antibodies make them ideal biomarkers. Additionally, antibodies are often elicited early in the ontogeny of different chronic and infectious diseases. We previously reported that antibodies from patients with infectious disease and separately those with Alzheimer's disease display a characteristic and reproducible "immunosignature" on a microarray of 10,000 random sequence peptides. Here we investigate the physical and chemical parameters underlying how immunosignaturing works. We first show that a variety of monoclonal and polyclonal antibodies raised against different classes of antigens produce distinct profiles on this microarray and the relative affinities are determined. A proposal for how antibodies bind the random sequences is tested. Sera from vaccinated mice and people suffering from a fugal infection are individually assayed to determine the complexity of signals that can be distinguished. Based on these results, we propose that this simple, general and inexpensive system could be optimized to generate a new class of antibody biomarkers for a wide variety of diseases.

Original languageEnglish (US)
JournalMolecular and Cellular Proteomics
Volume11
Issue number4
DOIs
StatePublished - Apr 2012

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Antibodies
Biomarkers
Communicable Diseases
Microarrays
Immunoglobulin Isotypes
Alzheimer Disease
Chronic Disease
Monoclonal Antibodies
Antigens
Peptides
Infection
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry
  • Medicine(all)

Cite this

Physical characterization of the "immunosignaturing effect". / Stafford, Phillip; Halperin, Rebecca; Legutki, Joseph Bart; Magee, Dewey Mitchell; Galgiani, John N; Johnston, Stephen Albert.

In: Molecular and Cellular Proteomics, Vol. 11, No. 4, 04.2012.

Research output: Contribution to journalArticle

Stafford, Phillip ; Halperin, Rebecca ; Legutki, Joseph Bart ; Magee, Dewey Mitchell ; Galgiani, John N ; Johnston, Stephen Albert. / Physical characterization of the "immunosignaturing effect". In: Molecular and Cellular Proteomics. 2012 ; Vol. 11, No. 4.
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