Physical mapping of the liguleless linkage group in sorghum bicolor using rice RFLP-selected sorghum BACs

Michael S. Zwick, M. Nurul Islam-Faridi, Don G. Czeschin, Rod A Wing, Gary E. Hart, David M. Stelly, H. James Price

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Physical mapping of BACs by fluorescent in situ hybridization (FISH) was used to analyze the liguleless (lg-1) linkage group in sorghum and compare it to the conserved region in rice and maize. Six liguleless-associated rice restriction fragment length polymorphism (RFLP) markers were used to select 16 homeologous sorghum BAGs, which were in turn used to physically map the liguleless linkage group in sorghum. Results show a basic conservation of the liguleless region in sorghum relative to the linkage map of rice. One marker which is distal in rice is more medial in sorghum, and another marker which is found within the linkage group in rice is on a different chromosome in sorghum. BACs associated with linkage group I hybridize to chromosome I(t), which was identified by using FISH in a sorghum cytogenetic stock trisomic for chromosome I (denoted I(t)), and a BAC associated with linkage group E hybridized to an unidentified chromosome. Selected BAGs, representing RFLP loci, were end-cloned for RFLP mapping, and the relative linkage order of these clones was in full agreement with the physical data. Similarities in locus order and the association of RFLP-selected BAC markers with two different chromosomes were found to exist between the linkage map of the liguleless region in maize and the physical map of the liguleless region in sorghum.

Original languageEnglish (US)
Pages (from-to)1983-1992
Number of pages10
JournalGenetics
Volume148
Issue number4
StatePublished - Apr 1998
Externally publishedYes

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Sorghum
Chromosome Mapping
Restriction Fragment Length Polymorphisms
Chromosomes
Fluorescence In Situ Hybridization
Zea mays
Oryza
Cytogenetics
Clone Cells

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Zwick, M. S., Islam-Faridi, M. N., Czeschin, D. G., Wing, R. A., Hart, G. E., Stelly, D. M., & Price, H. J. (1998). Physical mapping of the liguleless linkage group in sorghum bicolor using rice RFLP-selected sorghum BACs. Genetics, 148(4), 1983-1992.

Physical mapping of the liguleless linkage group in sorghum bicolor using rice RFLP-selected sorghum BACs. / Zwick, Michael S.; Islam-Faridi, M. Nurul; Czeschin, Don G.; Wing, Rod A; Hart, Gary E.; Stelly, David M.; Price, H. James.

In: Genetics, Vol. 148, No. 4, 04.1998, p. 1983-1992.

Research output: Contribution to journalArticle

Zwick, MS, Islam-Faridi, MN, Czeschin, DG, Wing, RA, Hart, GE, Stelly, DM & Price, HJ 1998, 'Physical mapping of the liguleless linkage group in sorghum bicolor using rice RFLP-selected sorghum BACs', Genetics, vol. 148, no. 4, pp. 1983-1992.
Zwick MS, Islam-Faridi MN, Czeschin DG, Wing RA, Hart GE, Stelly DM et al. Physical mapping of the liguleless linkage group in sorghum bicolor using rice RFLP-selected sorghum BACs. Genetics. 1998 Apr;148(4):1983-1992.
Zwick, Michael S. ; Islam-Faridi, M. Nurul ; Czeschin, Don G. ; Wing, Rod A ; Hart, Gary E. ; Stelly, David M. ; Price, H. James. / Physical mapping of the liguleless linkage group in sorghum bicolor using rice RFLP-selected sorghum BACs. In: Genetics. 1998 ; Vol. 148, No. 4. pp. 1983-1992.
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