Physiological Methods

Raina M. Maier, Terry J. Gentry

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Environmental microbiologists are not only interested in "who" is in the community, but also "what activities" the community carries out. Such activities can be identified and quantified via "physiological methods." The goal of this chapter is to examine different types of microbial activity measurements in both pure culture and environmental samples. In pure culture, activity measurements include: measurement of substrate disappearance; terminal electron acceptor (TEA) utilization; cell mass increase; and carbon dioxide evolution. Environmental activity measurements are discussed in terms of: carbon respiration; incorporation of radiolabeled tracers; adenylate energy determination; enzymatic assays; and stable isotope probing.

Original languageEnglish (US)
Title of host publicationEnvironmental Microbiology
Subtitle of host publicationThird Edition
PublisherElsevier Inc.
Pages213-243
Number of pages31
ISBN (Print)9780123946263
DOIs
StatePublished - Jan 1 2015

Keywords

  • ATP assays
  • Microbial carbon respiration
  • Microbial cell mass
  • Microbial substrate utilization
  • Microbial terminal electron acceptors
  • Physiological methods
  • Radiolabeled tracers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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