Physiology and morphology of protocerebral olfactory neurons in the male moth Manduca sexta

Ryohei Kanzaki, Edmund A. Arbas, John G Hildebrand

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

1. We have used intracellular recording and staining with Lucifer Yellow, followed by reconstruction from serial sections, to characterize the responses and structure of olfactory neurons in the protocerebrum (PC) of the brain of the male sphinx moth Manduca sexta. 2. Many olfactory protocerebral neurons (PCNs) innervate a particular neuropil region lateral to the central body, the lateral accessory lobe (LAL), which appears to be important for processing olfactory information. 3. Each LAL is linked by its constituent neurons to the ipsilateral lateral PC, where projection neurons from the antennal lobe terminate, as well as to other regions of the PC. The LALs are also linked to each other by bilateral neurons with arborizations in each LAL. 4. Some PC neurons showed long-lasting excitation (LLE) that outlasted the olfactory stimuli by ≥ 1 s, and as long as 30 s in some preparations. LLE was more frequently elicited by the sex-pheromone blend than by individual pheromone components. All bilateral neurons that showed LLE had arborizations in the LALs. LLE responses were also recorded in a single local neuron innervating the mushroom body. 5. In some other PC neurons, pheromonal stimuli elicited brief excitations that recovered to background firing rates <1 s after stimulation.

Original languageEnglish (US)
Pages (from-to)281-298
Number of pages18
JournalJournal of Comparative Physiology A
Volume168
Issue number3
DOIs
StatePublished - Mar 1991

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Manduca
Moths
Manduca sexta
moth
physiology
moths
neurons
Neurons
sex pheromone
mushroom
pheromone
brain
Mushroom Bodies
pheromone blends
antennal lobe
mushroom bodies
Sphingidae
Sex Attractants
Neuropil
Pheromones

Keywords

  • Manduca sexta
  • Moth
  • Olfactory interneurons
  • Pheromone
  • Protocerebrum

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Neuroscience(all)
  • Physiology (medical)
  • Physiology
  • Animal Science and Zoology

Cite this

Physiology and morphology of protocerebral olfactory neurons in the male moth Manduca sexta. / Kanzaki, Ryohei; Arbas, Edmund A.; Hildebrand, John G.

In: Journal of Comparative Physiology A, Vol. 168, No. 3, 03.1991, p. 281-298.

Research output: Contribution to journalArticle

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