picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome

L. Rong, S. J. Karcher, K. O'Neal, Martha C Hawes, C. D. Yerkes, R. K. Jayaswal, C. A. Hallberg, S. B. Gelvin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible chromosomal), harbored by A. tumefaciens At156, was inducible 10- to 50-fold by these extracts. Mutation of picA had no detectable effect upon bacterial growth or virulence under laboratory assay conditions. However, A. tumefaciens cells harboring a mutated picA locus aggregated into long 'ropes' when incubated with pea root tip cells. Such aggregation was not displayed by the parental strain A. tumefaciens A136. A preliminary characterization of the inducing compound in the carrot root extract suggests that the active substance is an acidic polysaccharide that is most likely derived from the pectic portion of the plant cell wall.

Original languageEnglish (US)
Pages (from-to)5828-5836
Number of pages9
JournalJournal of Bacteriology
Volume172
Issue number10
StatePublished - 1990
Externally publishedYes

Fingerprint

Agrobacterium tumefaciens
Chromosomes
Daucus carota
Meristem
Peas
Plant Cells
Cell Wall
Polysaccharides
Virulence
Mutation
Growth
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Rong, L., Karcher, S. J., O'Neal, K., Hawes, M. C., Yerkes, C. D., Jayaswal, R. K., ... Gelvin, S. B. (1990). picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome. Journal of Bacteriology, 172(10), 5828-5836.

picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome. / Rong, L.; Karcher, S. J.; O'Neal, K.; Hawes, Martha C; Yerkes, C. D.; Jayaswal, R. K.; Hallberg, C. A.; Gelvin, S. B.

In: Journal of Bacteriology, Vol. 172, No. 10, 1990, p. 5828-5836.

Research output: Contribution to journalArticle

Rong, L, Karcher, SJ, O'Neal, K, Hawes, MC, Yerkes, CD, Jayaswal, RK, Hallberg, CA & Gelvin, SB 1990, 'picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome', Journal of Bacteriology, vol. 172, no. 10, pp. 5828-5836.
Rong L, Karcher SJ, O'Neal K, Hawes MC, Yerkes CD, Jayaswal RK et al. picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome. Journal of Bacteriology. 1990;172(10):5828-5836.
Rong, L. ; Karcher, S. J. ; O'Neal, K. ; Hawes, Martha C ; Yerkes, C. D. ; Jayaswal, R. K. ; Hallberg, C. A. ; Gelvin, S. B. / picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome. In: Journal of Bacteriology. 1990 ; Vol. 172, No. 10. pp. 5828-5836.
@article{0674f51828004bedb198ff24b1298616,
title = "picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome",
abstract = "We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible chromosomal), harbored by A. tumefaciens At156, was inducible 10- to 50-fold by these extracts. Mutation of picA had no detectable effect upon bacterial growth or virulence under laboratory assay conditions. However, A. tumefaciens cells harboring a mutated picA locus aggregated into long 'ropes' when incubated with pea root tip cells. Such aggregation was not displayed by the parental strain A. tumefaciens A136. A preliminary characterization of the inducing compound in the carrot root extract suggests that the active substance is an acidic polysaccharide that is most likely derived from the pectic portion of the plant cell wall.",
author = "L. Rong and Karcher, {S. J.} and K. O'Neal and Hawes, {Martha C} and Yerkes, {C. D.} and Jayaswal, {R. K.} and Hallberg, {C. A.} and Gelvin, {S. B.}",
year = "1990",
language = "English (US)",
volume = "172",
pages = "5828--5836",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - picA, a novel plant-inducible locus on the Agrobacterium tumefaciens chromosome

AU - Rong, L.

AU - Karcher, S. J.

AU - O'Neal, K.

AU - Hawes, Martha C

AU - Yerkes, C. D.

AU - Jayaswal, R. K.

AU - Hallberg, C. A.

AU - Gelvin, S. B.

PY - 1990

Y1 - 1990

N2 - We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible chromosomal), harbored by A. tumefaciens At156, was inducible 10- to 50-fold by these extracts. Mutation of picA had no detectable effect upon bacterial growth or virulence under laboratory assay conditions. However, A. tumefaciens cells harboring a mutated picA locus aggregated into long 'ropes' when incubated with pea root tip cells. Such aggregation was not displayed by the parental strain A. tumefaciens A136. A preliminary characterization of the inducing compound in the carrot root extract suggests that the active substance is an acidic polysaccharide that is most likely derived from the pectic portion of the plant cell wall.

AB - We used the transposon Mu dI1681 to identify genes on the Agrobacterium tumefaciens chromosome that are inducible by extracts from carrot roots. One such locus (picA, for plant inducible chromosomal), harbored by A. tumefaciens At156, was inducible 10- to 50-fold by these extracts. Mutation of picA had no detectable effect upon bacterial growth or virulence under laboratory assay conditions. However, A. tumefaciens cells harboring a mutated picA locus aggregated into long 'ropes' when incubated with pea root tip cells. Such aggregation was not displayed by the parental strain A. tumefaciens A136. A preliminary characterization of the inducing compound in the carrot root extract suggests that the active substance is an acidic polysaccharide that is most likely derived from the pectic portion of the plant cell wall.

UR - http://www.scopus.com/inward/record.url?scp=0025163559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025163559&partnerID=8YFLogxK

M3 - Article

C2 - 2170328

AN - SCOPUS:0025163559

VL - 172

SP - 5828

EP - 5836

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 10

ER -