Piggybac-like elements in the pink bollworm, pectinophora gossypiella

J. Wang, E. D. Miller, G. S. Simmons, T. A. Miller, Bruce E Tabashnik, Y. Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A transgenic line of the pink bollworm, Pectinophora gossypiella, a key lepidopteran cotton pest, was generated previously using the piggyBac transposon IFP2 from Trichoplusia ni. Here we identified an endogenous piggyBac-like element (PLE), designated as PgPLE1, in the pink bollworm. A putatively intact copy of PgPLE1 (PgPLE1.1) presents the canonical features of PLE: inverted terminal repeats with three C/G residues at the extreme ends, inverted subterminal repeats, TTAA target site and an open reading frame encoding transposase with 68% similarity to IFP2. Vectorette PCR revealed large variation in the insertion sites of PgPLE1 amongst worldwide populations, indicating the potential mobility of PgPLE1. The PgPLE1 was undetectable in the genome of Pectinophora endema, implying the recent invasion of PgPLE1 after the divergence of these two closely related species.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalInsect Molecular Biology
Volume19
Issue number2
DOIs
StatePublished - Apr 2010

Fingerprint

Pectinophora gossypiella
Transposases
Terminal Repeat Sequences
Open Reading Frames
Genome
Polymerase Chain Reaction
Pectinophora
Population
Trichoplusia ni
terminal repeat sequences
transposons
open reading frames
cotton
Lepidoptera
pests
genetically modified organisms
genome

Keywords

  • Insertion site variation
  • Pectinophora gossypiella
  • PiggyBac
  • Pink bollworm
  • Transposon

ASJC Scopus subject areas

  • Insect Science
  • Molecular Biology
  • Genetics

Cite this

Piggybac-like elements in the pink bollworm, pectinophora gossypiella. / Wang, J.; Miller, E. D.; Simmons, G. S.; Miller, T. A.; Tabashnik, Bruce E; Park, Y.

In: Insect Molecular Biology, Vol. 19, No. 2, 04.2010, p. 177-184.

Research output: Contribution to journalArticle

Wang, J. ; Miller, E. D. ; Simmons, G. S. ; Miller, T. A. ; Tabashnik, Bruce E ; Park, Y. / Piggybac-like elements in the pink bollworm, pectinophora gossypiella. In: Insect Molecular Biology. 2010 ; Vol. 19, No. 2. pp. 177-184.
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