Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.

M. K. Taha, Magdalene "Maggie" So, H. S. Seifert, E. Billyard, C. Marchal

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

We have identified two closely linked genes, pilA and pilB, which act in trans on the pilin promoter. pilA-pilB map downstream of expression loci pilE1 and opaE1 in the gonococcal chromosome. Subcloning data indicate that pilB acts negatively on the pilin promoter, and insertional inactivation of pilB results in hyperpiliated gonococci. A pilA clone activates the pilin promoter in Escherichia coli, and a pilA-/pilA+ heterodiploid gonococcus exhibits a P- phenotype. Our inability to obtain simple pilA- mutants strongly suggests that pilA is an essential gene in the gonococcus. In an in vitro coupled transcription/translation system, inserts spanning the pilA and pilB region direct the synthesis of two proteins of 40 and 58 kd. DNA sequence analysis shows that the pilA and pilB loci encode proteins of 38.6 kd (with a putative DNA binding domain) and 57.9 kd respectively. The pilA and pilB genes are in opposite orientation relative to each other, and the 5' ends of the two genes overlap. We discuss how these two loci may interact to control pilin expression in the gonococcus.

Original languageEnglish (US)
Pages (from-to)4367-4378
Number of pages12
JournalEMBO Journal
Volume7
Issue number13
StatePublished - Dec 20 1988
Externally publishedYes

Fingerprint

Fimbriae Proteins
Neisseria gonorrhoeae
Genes
DNA sequences
Essential Genes
Transcription
Chromosomes
DNA Sequence Analysis
Escherichia coli
Proteins
Clone Cells
Phenotype
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Taha, M. K., So, M. M., Seifert, H. S., Billyard, E., & Marchal, C. (1988). Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control. EMBO Journal, 7(13), 4367-4378.

Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control. / Taha, M. K.; So, Magdalene "Maggie"; Seifert, H. S.; Billyard, E.; Marchal, C.

In: EMBO Journal, Vol. 7, No. 13, 20.12.1988, p. 4367-4378.

Research output: Contribution to journalArticle

Taha, MK, So, MM, Seifert, HS, Billyard, E & Marchal, C 1988, 'Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.', EMBO Journal, vol. 7, no. 13, pp. 4367-4378.
Taha, M. K. ; So, Magdalene "Maggie" ; Seifert, H. S. ; Billyard, E. ; Marchal, C. / Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control. In: EMBO Journal. 1988 ; Vol. 7, No. 13. pp. 4367-4378.
@article{1e3740ea7af042c39d81150d1e0cb946,
title = "Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.",
abstract = "We have identified two closely linked genes, pilA and pilB, which act in trans on the pilin promoter. pilA-pilB map downstream of expression loci pilE1 and opaE1 in the gonococcal chromosome. Subcloning data indicate that pilB acts negatively on the pilin promoter, and insertional inactivation of pilB results in hyperpiliated gonococci. A pilA clone activates the pilin promoter in Escherichia coli, and a pilA-/pilA+ heterodiploid gonococcus exhibits a P- phenotype. Our inability to obtain simple pilA- mutants strongly suggests that pilA is an essential gene in the gonococcus. In an in vitro coupled transcription/translation system, inserts spanning the pilA and pilB region direct the synthesis of two proteins of 40 and 58 kd. DNA sequence analysis shows that the pilA and pilB loci encode proteins of 38.6 kd (with a putative DNA binding domain) and 57.9 kd respectively. The pilA and pilB genes are in opposite orientation relative to each other, and the 5' ends of the two genes overlap. We discuss how these two loci may interact to control pilin expression in the gonococcus.",
author = "Taha, {M. K.} and So, {Magdalene {"}Maggie{"}} and Seifert, {H. S.} and E. Billyard and C. Marchal",
year = "1988",
month = "12",
day = "20",
language = "English (US)",
volume = "7",
pages = "4367--4378",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "13",

}

TY - JOUR

T1 - Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.

AU - Taha, M. K.

AU - So, Magdalene "Maggie"

AU - Seifert, H. S.

AU - Billyard, E.

AU - Marchal, C.

PY - 1988/12/20

Y1 - 1988/12/20

N2 - We have identified two closely linked genes, pilA and pilB, which act in trans on the pilin promoter. pilA-pilB map downstream of expression loci pilE1 and opaE1 in the gonococcal chromosome. Subcloning data indicate that pilB acts negatively on the pilin promoter, and insertional inactivation of pilB results in hyperpiliated gonococci. A pilA clone activates the pilin promoter in Escherichia coli, and a pilA-/pilA+ heterodiploid gonococcus exhibits a P- phenotype. Our inability to obtain simple pilA- mutants strongly suggests that pilA is an essential gene in the gonococcus. In an in vitro coupled transcription/translation system, inserts spanning the pilA and pilB region direct the synthesis of two proteins of 40 and 58 kd. DNA sequence analysis shows that the pilA and pilB loci encode proteins of 38.6 kd (with a putative DNA binding domain) and 57.9 kd respectively. The pilA and pilB genes are in opposite orientation relative to each other, and the 5' ends of the two genes overlap. We discuss how these two loci may interact to control pilin expression in the gonococcus.

AB - We have identified two closely linked genes, pilA and pilB, which act in trans on the pilin promoter. pilA-pilB map downstream of expression loci pilE1 and opaE1 in the gonococcal chromosome. Subcloning data indicate that pilB acts negatively on the pilin promoter, and insertional inactivation of pilB results in hyperpiliated gonococci. A pilA clone activates the pilin promoter in Escherichia coli, and a pilA-/pilA+ heterodiploid gonococcus exhibits a P- phenotype. Our inability to obtain simple pilA- mutants strongly suggests that pilA is an essential gene in the gonococcus. In an in vitro coupled transcription/translation system, inserts spanning the pilA and pilB region direct the synthesis of two proteins of 40 and 58 kd. DNA sequence analysis shows that the pilA and pilB loci encode proteins of 38.6 kd (with a putative DNA binding domain) and 57.9 kd respectively. The pilA and pilB genes are in opposite orientation relative to each other, and the 5' ends of the two genes overlap. We discuss how these two loci may interact to control pilin expression in the gonococcus.

UR - http://www.scopus.com/inward/record.url?scp=0024293537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024293537&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 4367

EP - 4378

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 13

ER -