Pilin regulation in the pilT mutant of Neisseria gonorrhoeae strain MS11

Manuela Dietrich, Hans Mollenkopf, Magdalene "Maggie" So, Alexandra Friedrich

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The ATPase protein PilT mediates retraction of type IV pili (Tfp). Tfp retraction of Neisseria gonorrhoeae causes many signal transduction events and changes in gene expression in infected epithelial cells. To find out whether a pilT mutation and lack of Tfp retraction, respectively, lead also to gene regulation in bacteria, we performed microarrays comparing the transcriptional profiles of the N. gonorrhoeae parent strain MS11 and its isogenic pilT mutant during growth in vitro. A loss-of-function-mutation in pilT led to altered transcript levels of 63 ORFs. Levels of pilE transcripts and its deduced protein, the major Tfp subunit pilin, were increased most markedly by a mutation in pilT. Further studies revealed that pilE expression was also controlled by two other genes encoding Tfp biogenesis proteins, pilD and pilF. Our studies strongly suggest that pilE expression is a finely tuned process.

Original languageEnglish (US)
Pages (from-to)248-256
Number of pages9
JournalFEMS Microbiology Letters
Volume296
Issue number2
DOIs
StatePublished - Jul 2009

Fingerprint

Fimbriae Proteins
Neisseria gonorrhoeae
Mutation
Proteins
Open Reading Frames
Genes
Adenosine Triphosphatases
Signal Transduction
Epithelial Cells
Bacteria
Gene Expression
Growth

Keywords

  • Neisseria gonorrhoeae
  • PilE expression
  • PilT-responsive genes

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Pilin regulation in the pilT mutant of Neisseria gonorrhoeae strain MS11. / Dietrich, Manuela; Mollenkopf, Hans; So, Magdalene "Maggie"; Friedrich, Alexandra.

In: FEMS Microbiology Letters, Vol. 296, No. 2, 07.2009, p. 248-256.

Research output: Contribution to journalArticle

Dietrich, Manuela ; Mollenkopf, Hans ; So, Magdalene "Maggie" ; Friedrich, Alexandra. / Pilin regulation in the pilT mutant of Neisseria gonorrhoeae strain MS11. In: FEMS Microbiology Letters. 2009 ; Vol. 296, No. 2. pp. 248-256.
@article{59d1b07ec9274a309c29ee8a68a51429,
title = "Pilin regulation in the pilT mutant of Neisseria gonorrhoeae strain MS11",
abstract = "The ATPase protein PilT mediates retraction of type IV pili (Tfp). Tfp retraction of Neisseria gonorrhoeae causes many signal transduction events and changes in gene expression in infected epithelial cells. To find out whether a pilT mutation and lack of Tfp retraction, respectively, lead also to gene regulation in bacteria, we performed microarrays comparing the transcriptional profiles of the N. gonorrhoeae parent strain MS11 and its isogenic pilT mutant during growth in vitro. A loss-of-function-mutation in pilT led to altered transcript levels of 63 ORFs. Levels of pilE transcripts and its deduced protein, the major Tfp subunit pilin, were increased most markedly by a mutation in pilT. Further studies revealed that pilE expression was also controlled by two other genes encoding Tfp biogenesis proteins, pilD and pilF. Our studies strongly suggest that pilE expression is a finely tuned process.",
keywords = "Neisseria gonorrhoeae, PilE expression, PilT-responsive genes",
author = "Manuela Dietrich and Hans Mollenkopf and So, {Magdalene {"}Maggie{"}} and Alexandra Friedrich",
year = "2009",
month = "7",
doi = "10.1111/j.1574-6968.2009.01647.x",
language = "English (US)",
volume = "296",
pages = "248--256",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Pilin regulation in the pilT mutant of Neisseria gonorrhoeae strain MS11

AU - Dietrich, Manuela

AU - Mollenkopf, Hans

AU - So, Magdalene "Maggie"

AU - Friedrich, Alexandra

PY - 2009/7

Y1 - 2009/7

N2 - The ATPase protein PilT mediates retraction of type IV pili (Tfp). Tfp retraction of Neisseria gonorrhoeae causes many signal transduction events and changes in gene expression in infected epithelial cells. To find out whether a pilT mutation and lack of Tfp retraction, respectively, lead also to gene regulation in bacteria, we performed microarrays comparing the transcriptional profiles of the N. gonorrhoeae parent strain MS11 and its isogenic pilT mutant during growth in vitro. A loss-of-function-mutation in pilT led to altered transcript levels of 63 ORFs. Levels of pilE transcripts and its deduced protein, the major Tfp subunit pilin, were increased most markedly by a mutation in pilT. Further studies revealed that pilE expression was also controlled by two other genes encoding Tfp biogenesis proteins, pilD and pilF. Our studies strongly suggest that pilE expression is a finely tuned process.

AB - The ATPase protein PilT mediates retraction of type IV pili (Tfp). Tfp retraction of Neisseria gonorrhoeae causes many signal transduction events and changes in gene expression in infected epithelial cells. To find out whether a pilT mutation and lack of Tfp retraction, respectively, lead also to gene regulation in bacteria, we performed microarrays comparing the transcriptional profiles of the N. gonorrhoeae parent strain MS11 and its isogenic pilT mutant during growth in vitro. A loss-of-function-mutation in pilT led to altered transcript levels of 63 ORFs. Levels of pilE transcripts and its deduced protein, the major Tfp subunit pilin, were increased most markedly by a mutation in pilT. Further studies revealed that pilE expression was also controlled by two other genes encoding Tfp biogenesis proteins, pilD and pilF. Our studies strongly suggest that pilE expression is a finely tuned process.

KW - Neisseria gonorrhoeae

KW - PilE expression

KW - PilT-responsive genes

UR - http://www.scopus.com/inward/record.url?scp=67649233384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649233384&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2009.01647.x

DO - 10.1111/j.1574-6968.2009.01647.x

M3 - Article

C2 - 19486161

AN - SCOPUS:67649233384

VL - 296

SP - 248

EP - 256

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -