Pillar size effect on DNA electrophoresis in microchips with sub-micron pillar arrays

Yick Chuen Chan, Yi Kuen Lee, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper presents a systematic study of DNA electrophoresis in microchannels integrated with sub-micron pillar arrays. Electrophoretic mobility of different DNA fragments in channels having pillars of various dimensions is measured. In addition to confirming a previously observed nonlinear relationship with applied electric field following the Biased Reptation with Fluctuations Model, the mobility dependence on DNA size and pillar spacing is characterized and discussed. Similar to conventional slab-gel electrophoresis, short DNA molecules have higher migration mobility. However, a "band inversion"-like phenomenon is observed for larger DNA molecules. The pillar array significantly affects the electric field distribution in the separation channel and, consequently, the resulting mobility. Although this leads to a higher mobility in more closely packed pillars, excessive reduction of the pillar spacing generates a large retarding force. This counteracts the effect of electric field on the overall DNA mobility.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages413-416
Number of pages4
StatePublished - 2007
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: Jan 21 2007Jan 25 2007

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period1/21/071/25/07

Fingerprint

electrophoresis
Electrophoresis
DNA
deoxyribonucleic acid
Electric fields
electric fields
spacing
Electrophoretic mobility
Molecules
microchannels
Microchannels
molecules
slabs
Gels
fragments
retarding
gels
inversions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Chan, Y. C., Lee, Y. K., & Zohar, Y. (2007). Pillar size effect on DNA electrophoresis in microchips with sub-micron pillar arrays. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 413-416). [4432978]

Pillar size effect on DNA electrophoresis in microchips with sub-micron pillar arrays. / Chan, Yick Chuen; Lee, Yi Kuen; Zohar, Yitshak.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. p. 413-416 4432978.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chan, YC, Lee, YK & Zohar, Y 2007, Pillar size effect on DNA electrophoresis in microchips with sub-micron pillar arrays. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 4432978, pp. 413-416, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 1/21/07.
Chan YC, Lee YK, Zohar Y. Pillar size effect on DNA electrophoresis in microchips with sub-micron pillar arrays. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. p. 413-416. 4432978
Chan, Yick Chuen ; Lee, Yi Kuen ; Zohar, Yitshak. / Pillar size effect on DNA electrophoresis in microchips with sub-micron pillar arrays. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2007. pp. 413-416
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