Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells

Ningfei An, Ying Wei Lin, Sandeep Mahajan, Joshua N. Kellner, Yong Wang, Zihai Li, Andrew Kraft, Yubin Kang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The genes and pathways that govern the functions and expansion of hematopoietic stem cells (HSC) are not completely understood. In this study, we investigated the roles of serine/threonine Pim kinases in hematopoiesis in mice. We generated PIM1 transgenic mice (Pim1-Tx) overexpressing human PIM1 driven by vav hematopoietic promoter/regulatory elements. Compared to wild-type littermates, Pim1-Tx mice showed enhanced hematopoiesis as demonstrated by increased numbers of Lin-Sca-1+ c-Kit+ (LSK) hematopoietic stem/progenitor cells and cobblestone area forming cells, higher BrdU incorporation in long-term HSC population, and a better ability to reconstitute lethally irradiated mice. We then extended our study using Pim1-/-, Pim2-/-, Pim3-/- single knockout (KO) mice. HSCs from Pim1-/- KO mice showed impaired long-term hematopoietic repopulating capacity in secondary and competitive transplantations. Interestingly, these defects were not observed in HSCs from Pim2-/- or Pim3-/- KO mice. Limiting dilution competitive transplantation assay estimated that the frequency of LSKCD34- HSCs was reduced by approximately 28-fold in Pim1-/- KO mice compared to wild-type littermates. Mechanistic studies demonstrated an important role of Pim1 kinase in regulating HSC cell proliferation and survival. Finally, our polymerase chain reaction (PCR) array and confirmatory real-time PCR (RT-PCR) studies identified several genes including Lef-1, Pax5, and Gata1 in HSCs that were affected by Pim1 deletion. Our data provide the first direct evidence for the important role of Pim1 kinase in the regulation of HSCs. Our study also dissects out the relative role of individual Pim kinase in HSC functions and regulation.

Original languageEnglish (US)
Pages (from-to)1202-1212
Number of pages11
JournalStem Cells
Volume31
Issue number6
DOIs
StatePublished - Jun 2013
Externally publishedYes

Fingerprint

Protein-Serine-Threonine Kinases
Hematopoietic Stem Cells
Knockout Mice
Hematopoiesis
Phosphotransferases
Transplantation
Bromodeoxyuridine
Transgenic Mice
Genes
Real-Time Polymerase Chain Reaction
Cell Survival
Cell Proliferation
Polymerase Chain Reaction
Population

Keywords

  • Apoptosis
  • Hematopoietic stem cell transplantation
  • Hematopoietic stem cells
  • Knockout mouse
  • Pim kinase
  • Proliferation
  • Serine/threonine kinase
  • Transgenic mouse

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

An, N., Lin, Y. W., Mahajan, S., Kellner, J. N., Wang, Y., Li, Z., ... Kang, Y. (2013). Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells. Stem Cells, 31(6), 1202-1212. https://doi.org/10.1002/stem.1369

Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells. / An, Ningfei; Lin, Ying Wei; Mahajan, Sandeep; Kellner, Joshua N.; Wang, Yong; Li, Zihai; Kraft, Andrew; Kang, Yubin.

In: Stem Cells, Vol. 31, No. 6, 06.2013, p. 1202-1212.

Research output: Contribution to journalArticle

An, N, Lin, YW, Mahajan, S, Kellner, JN, Wang, Y, Li, Z, Kraft, A & Kang, Y 2013, 'Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells', Stem Cells, vol. 31, no. 6, pp. 1202-1212. https://doi.org/10.1002/stem.1369
An, Ningfei ; Lin, Ying Wei ; Mahajan, Sandeep ; Kellner, Joshua N. ; Wang, Yong ; Li, Zihai ; Kraft, Andrew ; Kang, Yubin. / Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells. In: Stem Cells. 2013 ; Vol. 31, No. 6. pp. 1202-1212.
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