Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20

Ling Wang, Yuemin Ma, Xueqin Guo, Peng Wan, Kaiyu Liu, Shengbo Cong, Jintao Wang, Dong Xu, Yutao Xiao, Xianchun Li, Bruce E Tabashnik, Kongming Wu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Insecticidal proteins from Bacillus thuringiensis (Bt) are widely used to control insect pests, but their efficacy is reduced when pests evolve resistance. We report on a novel allele (r16) of the cadherin gene (PgCad1) in pink bollworm (Pectinophora gossypiella) associated with resistance to Bt toxin Cry1Ac, which is produced by transgenic cotton. The r16 allele isolated from a field population in China has 1545 base pairs of a degenerate transposon inserted in exon 20 of PgCad1, which generates a mis-spliced transcript containing a premature stop codon. A strain homozygous for r16 had 300-fold resistance to Cry1Ac, 2.6-fold cross-resistance to Cry2Ab, and completed its life cycle on transgenic Bt cotton producing Cry1Ac. Inheritance of Cry1Ac resistance was recessive and tightly linked with r16. Compared with transfected insect cells expressing wild-type PgCad1, cells expressing r16 were less susceptible to Cry1Ac. Recombinant cadherin protein was transported to the cell membrane in cells transfected with the wild-type PgCad1 allele, but not in cells transfected with r16. Cadherin occurred on brush border membrane vesicles (BBMVs) in the midgut of susceptible larvae, but not resistant larvae. These results imply that the r16 allele mediates Cry1Ac resistance in pink bollworm by interfering with the localization of cadherin.

Original languageEnglish (US)
JournalToxins
Volume11
Issue number4
DOIs
StatePublished - Mar 28 2019

Fingerprint

Bacillus thuringiensis
Bacilli
Cadherins
Exons
Alleles
Cotton
Larva
Insect control
Cells
Insect Control
Recombinant proteins
Pest Control
Nonsense Codon
Brushes
Cell membranes
Microvilli
Life Cycle Stages
Recombinant Proteins
Base Pairing
Insects

Keywords

  • Bacillus thuringiensis
  • Bt cotton
  • Cry1Ac
  • genetically engineered crop
  • Pectinophora gossypiella
  • resistance mechanism
  • transposon

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20. / Wang, Ling; Ma, Yuemin; Guo, Xueqin; Wan, Peng; Liu, Kaiyu; Cong, Shengbo; Wang, Jintao; Xu, Dong; Xiao, Yutao; Li, Xianchun; Tabashnik, Bruce E; Wu, Kongming.

In: Toxins, Vol. 11, No. 4, 28.03.2019.

Research output: Contribution to journalArticle

Wang, L, Ma, Y, Guo, X, Wan, P, Liu, K, Cong, S, Wang, J, Xu, D, Xiao, Y, Li, X, Tabashnik, BE & Wu, K 2019, 'Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20', Toxins, vol. 11, no. 4. https://doi.org/10.3390/toxins11040186
Wang, Ling ; Ma, Yuemin ; Guo, Xueqin ; Wan, Peng ; Liu, Kaiyu ; Cong, Shengbo ; Wang, Jintao ; Xu, Dong ; Xiao, Yutao ; Li, Xianchun ; Tabashnik, Bruce E ; Wu, Kongming. / Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20. In: Toxins. 2019 ; Vol. 11, No. 4.
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AU - Cong, Shengbo

AU - Wang, Jintao

AU - Xu, Dong

AU - Xiao, Yutao

AU - Li, Xianchun

AU - Tabashnik, Bruce E

AU - Wu, Kongming

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N2 - Insecticidal proteins from Bacillus thuringiensis (Bt) are widely used to control insect pests, but their efficacy is reduced when pests evolve resistance. We report on a novel allele (r16) of the cadherin gene (PgCad1) in pink bollworm (Pectinophora gossypiella) associated with resistance to Bt toxin Cry1Ac, which is produced by transgenic cotton. The r16 allele isolated from a field population in China has 1545 base pairs of a degenerate transposon inserted in exon 20 of PgCad1, which generates a mis-spliced transcript containing a premature stop codon. A strain homozygous for r16 had 300-fold resistance to Cry1Ac, 2.6-fold cross-resistance to Cry2Ab, and completed its life cycle on transgenic Bt cotton producing Cry1Ac. Inheritance of Cry1Ac resistance was recessive and tightly linked with r16. Compared with transfected insect cells expressing wild-type PgCad1, cells expressing r16 were less susceptible to Cry1Ac. Recombinant cadherin protein was transported to the cell membrane in cells transfected with the wild-type PgCad1 allele, but not in cells transfected with r16. Cadherin occurred on brush border membrane vesicles (BBMVs) in the midgut of susceptible larvae, but not resistant larvae. These results imply that the r16 allele mediates Cry1Ac resistance in pink bollworm by interfering with the localization of cadherin.

AB - Insecticidal proteins from Bacillus thuringiensis (Bt) are widely used to control insect pests, but their efficacy is reduced when pests evolve resistance. We report on a novel allele (r16) of the cadherin gene (PgCad1) in pink bollworm (Pectinophora gossypiella) associated with resistance to Bt toxin Cry1Ac, which is produced by transgenic cotton. The r16 allele isolated from a field population in China has 1545 base pairs of a degenerate transposon inserted in exon 20 of PgCad1, which generates a mis-spliced transcript containing a premature stop codon. A strain homozygous for r16 had 300-fold resistance to Cry1Ac, 2.6-fold cross-resistance to Cry2Ab, and completed its life cycle on transgenic Bt cotton producing Cry1Ac. Inheritance of Cry1Ac resistance was recessive and tightly linked with r16. Compared with transfected insect cells expressing wild-type PgCad1, cells expressing r16 were less susceptible to Cry1Ac. Recombinant cadherin protein was transported to the cell membrane in cells transfected with the wild-type PgCad1 allele, but not in cells transfected with r16. Cadherin occurred on brush border membrane vesicles (BBMVs) in the midgut of susceptible larvae, but not resistant larvae. These results imply that the r16 allele mediates Cry1Ac resistance in pink bollworm by interfering with the localization of cadherin.

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