Planar imaging of 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) can detect resting ischemia

Gerald Johnson, Kiem N. Nguyen, Zhonglin Liu, Ping Gao, Roberto Pasqualini, Robert D. Okada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background. 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) (99mTcN-NOET) is a new lipophilic, neutral-charge cardiac perfusion imaging agent that demonstrates apparent redistribution in animal models and humans. The purpose of this study was to determine whether the kinetics of 99mTcN-NOET are suitable for the detection of resting ischemia. Methods and Results. Microspheres were injected at baseline and simultaneously with 99mTcN-NOET after a 90% reduction in resting how in the left circumflex coronary artery in six open-chest canine experiments. The relationship of flow and activity early after injection was determined in one experiment by termination at 10 minutes. The flow ratio (left circumflex/left anterior descending coronary artery) after stenosis fell significantly (0.87 ± 0.04 vs 0.46 ± 0.04; p < 0.05). The end-tissue 99mTc ratio (0.78 ± 0.05) was significantly higher than the flow ratio at injection (0.46 ± 0.04; p < 0.05), indicating substantial redistribution. In vivo imaging was conducted during 2 hours in five experiments, followed by ex vivo imaging. Myocardial clearance from 10 minutes onward was biphasic in left anterior descending and monophasic in left circumflex coronary arteries. Myocardial clearance from 10 to 60 minutes was delayed in left circumflex (35.5% ± 8.1%) versus left anterior descending coronary arteries (49.2% ± 8.6%; p < 0.05). No significant difference was observed from 60- to 120-minute clearance. Five of five experiments demonstrated initial defects and complete fill-in at 90 to 120 minutes by qualitative assessment. Quantitation of ex vivo images confirmed significant redistribution. Conclusions. Resting ischemia caused by moderate to severe stenosis can be detected on scans with 99mTcN-NOET. Redistribution was near complete in this model by 90 to 120 minutes. 99mTcN-NOET is a promising new agent for the detection of coronary artery disease in viable myocardium and warrants further investigation.

Original languageEnglish (US)
Pages (from-to)217-225
Number of pages9
JournalJournal of Nuclear Cardiology
Volume4
Issue number3
DOIs
StatePublished - May 1997
Externally publishedYes

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Technetium
Coronary Vessels
Ischemia
Injections
Perfusion Imaging
Coronary Stenosis
Microspheres
Canidae
Coronary Artery Disease
Myocardium
Pathologic Constriction
Thorax
Animal Models

Keywords

  • Tc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V))
  • Myocardium
  • Planar imaging
  • Redistribution

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Planar imaging of 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) can detect resting ischemia. / Johnson, Gerald; Nguyen, Kiem N.; Liu, Zhonglin; Gao, Ping; Pasqualini, Roberto; Okada, Robert D.

In: Journal of Nuclear Cardiology, Vol. 4, No. 3, 05.1997, p. 217-225.

Research output: Contribution to journalArticle

Johnson, Gerald ; Nguyen, Kiem N. ; Liu, Zhonglin ; Gao, Ping ; Pasqualini, Roberto ; Okada, Robert D. / Planar imaging of 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) can detect resting ischemia. In: Journal of Nuclear Cardiology. 1997 ; Vol. 4, No. 3. pp. 217-225.
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abstract = "Background. 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) (99mTcN-NOET) is a new lipophilic, neutral-charge cardiac perfusion imaging agent that demonstrates apparent redistribution in animal models and humans. The purpose of this study was to determine whether the kinetics of 99mTcN-NOET are suitable for the detection of resting ischemia. Methods and Results. Microspheres were injected at baseline and simultaneously with 99mTcN-NOET after a 90{\%} reduction in resting how in the left circumflex coronary artery in six open-chest canine experiments. The relationship of flow and activity early after injection was determined in one experiment by termination at 10 minutes. The flow ratio (left circumflex/left anterior descending coronary artery) after stenosis fell significantly (0.87 ± 0.04 vs 0.46 ± 0.04; p < 0.05). The end-tissue 99mTc ratio (0.78 ± 0.05) was significantly higher than the flow ratio at injection (0.46 ± 0.04; p < 0.05), indicating substantial redistribution. In vivo imaging was conducted during 2 hours in five experiments, followed by ex vivo imaging. Myocardial clearance from 10 minutes onward was biphasic in left anterior descending and monophasic in left circumflex coronary arteries. Myocardial clearance from 10 to 60 minutes was delayed in left circumflex (35.5{\%} ± 8.1{\%}) versus left anterior descending coronary arteries (49.2{\%} ± 8.6{\%}; p < 0.05). No significant difference was observed from 60- to 120-minute clearance. Five of five experiments demonstrated initial defects and complete fill-in at 90 to 120 minutes by qualitative assessment. Quantitation of ex vivo images confirmed significant redistribution. Conclusions. Resting ischemia caused by moderate to severe stenosis can be detected on scans with 99mTcN-NOET. Redistribution was near complete in this model by 90 to 120 minutes. 99mTcN-NOET is a promising new agent for the detection of coronary artery disease in viable myocardium and warrants further investigation.",
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T1 - Planar imaging of 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) can detect resting ischemia

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AU - Nguyen, Kiem N.

AU - Liu, Zhonglin

AU - Gao, Ping

AU - Pasqualini, Roberto

AU - Okada, Robert D.

PY - 1997/5

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N2 - Background. 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) (99mTcN-NOET) is a new lipophilic, neutral-charge cardiac perfusion imaging agent that demonstrates apparent redistribution in animal models and humans. The purpose of this study was to determine whether the kinetics of 99mTcN-NOET are suitable for the detection of resting ischemia. Methods and Results. Microspheres were injected at baseline and simultaneously with 99mTcN-NOET after a 90% reduction in resting how in the left circumflex coronary artery in six open-chest canine experiments. The relationship of flow and activity early after injection was determined in one experiment by termination at 10 minutes. The flow ratio (left circumflex/left anterior descending coronary artery) after stenosis fell significantly (0.87 ± 0.04 vs 0.46 ± 0.04; p < 0.05). The end-tissue 99mTc ratio (0.78 ± 0.05) was significantly higher than the flow ratio at injection (0.46 ± 0.04; p < 0.05), indicating substantial redistribution. In vivo imaging was conducted during 2 hours in five experiments, followed by ex vivo imaging. Myocardial clearance from 10 minutes onward was biphasic in left anterior descending and monophasic in left circumflex coronary arteries. Myocardial clearance from 10 to 60 minutes was delayed in left circumflex (35.5% ± 8.1%) versus left anterior descending coronary arteries (49.2% ± 8.6%; p < 0.05). No significant difference was observed from 60- to 120-minute clearance. Five of five experiments demonstrated initial defects and complete fill-in at 90 to 120 minutes by qualitative assessment. Quantitation of ex vivo images confirmed significant redistribution. Conclusions. Resting ischemia caused by moderate to severe stenosis can be detected on scans with 99mTcN-NOET. Redistribution was near complete in this model by 90 to 120 minutes. 99mTcN-NOET is a promising new agent for the detection of coronary artery disease in viable myocardium and warrants further investigation.

AB - Background. 99mTc-labeled (bis(N-ethoxy, N-ethyl dithiocarbamato) nitrido technetium(V)) (99mTcN-NOET) is a new lipophilic, neutral-charge cardiac perfusion imaging agent that demonstrates apparent redistribution in animal models and humans. The purpose of this study was to determine whether the kinetics of 99mTcN-NOET are suitable for the detection of resting ischemia. Methods and Results. Microspheres were injected at baseline and simultaneously with 99mTcN-NOET after a 90% reduction in resting how in the left circumflex coronary artery in six open-chest canine experiments. The relationship of flow and activity early after injection was determined in one experiment by termination at 10 minutes. The flow ratio (left circumflex/left anterior descending coronary artery) after stenosis fell significantly (0.87 ± 0.04 vs 0.46 ± 0.04; p < 0.05). The end-tissue 99mTc ratio (0.78 ± 0.05) was significantly higher than the flow ratio at injection (0.46 ± 0.04; p < 0.05), indicating substantial redistribution. In vivo imaging was conducted during 2 hours in five experiments, followed by ex vivo imaging. Myocardial clearance from 10 minutes onward was biphasic in left anterior descending and monophasic in left circumflex coronary arteries. Myocardial clearance from 10 to 60 minutes was delayed in left circumflex (35.5% ± 8.1%) versus left anterior descending coronary arteries (49.2% ± 8.6%; p < 0.05). No significant difference was observed from 60- to 120-minute clearance. Five of five experiments demonstrated initial defects and complete fill-in at 90 to 120 minutes by qualitative assessment. Quantitation of ex vivo images confirmed significant redistribution. Conclusions. Resting ischemia caused by moderate to severe stenosis can be detected on scans with 99mTcN-NOET. Redistribution was near complete in this model by 90 to 120 minutes. 99mTcN-NOET is a promising new agent for the detection of coronary artery disease in viable myocardium and warrants further investigation.

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