Planar supported bilayer polymers formed from bis-diene lipids by Langmuir-Blodgett deposition and UV irradiation

John C. Conboy, Sanchao Liu, David F. O'Brien, Steven S Saavedra

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Substrate-supported lipid bilayers have been prepared from bis-diene functionalized phosphorylcholine (PC) lipids and polymerized by UV irradiation. The overall bilayer structure is largely preserved upon removal from water, although significant loss of material occurs from the upper leaflet of the bilayer, likely due to desorption at the air/water interface. The morphology and surface structure of the bilayer, as observed by AFM, indicate a substantially different arrangement of the lipids in the hydrated and dehydrated states, presumably due to the loss of water from the near surface region. These changes have been correlated with infrared spectral shifts sensitive to the conformation of the hydrocarbon chains. Protein adsorption studies show that rehydrated, polymerized bilayers retain a degree of resistance to BSA adsorption intermediate between model hydrophobic and fluid PC lipid bilayer surfaces. The degree of protein adsorption is correlated with desorption of material from the upper leaflet of the bilayer upon drying, which produces voids at which hydrophobically driven protein adsorption occurs.

Original languageEnglish (US)
Pages (from-to)841-849
Number of pages9
JournalBiomacromolecules
Volume4
Issue number3
DOIs
StatePublished - May 2003

Fingerprint

Lipids
Adsorption
Polymers
Irradiation
Lipid bilayers
Phosphorylcholine
Lipid Bilayers
Proteins
Water
Desorption
Hydrocarbons
Surface structure
Conformations
Drying
Air
Infrared radiation
Fluids
Substrates

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Planar supported bilayer polymers formed from bis-diene lipids by Langmuir-Blodgett deposition and UV irradiation. / Conboy, John C.; Liu, Sanchao; O'Brien, David F.; Saavedra, Steven S.

In: Biomacromolecules, Vol. 4, No. 3, 05.2003, p. 841-849.

Research output: Contribution to journalArticle

@article{387289b22e4747309607a701b10bbea5,
title = "Planar supported bilayer polymers formed from bis-diene lipids by Langmuir-Blodgett deposition and UV irradiation",
abstract = "Substrate-supported lipid bilayers have been prepared from bis-diene functionalized phosphorylcholine (PC) lipids and polymerized by UV irradiation. The overall bilayer structure is largely preserved upon removal from water, although significant loss of material occurs from the upper leaflet of the bilayer, likely due to desorption at the air/water interface. The morphology and surface structure of the bilayer, as observed by AFM, indicate a substantially different arrangement of the lipids in the hydrated and dehydrated states, presumably due to the loss of water from the near surface region. These changes have been correlated with infrared spectral shifts sensitive to the conformation of the hydrocarbon chains. Protein adsorption studies show that rehydrated, polymerized bilayers retain a degree of resistance to BSA adsorption intermediate between model hydrophobic and fluid PC lipid bilayer surfaces. The degree of protein adsorption is correlated with desorption of material from the upper leaflet of the bilayer upon drying, which produces voids at which hydrophobically driven protein adsorption occurs.",
author = "Conboy, {John C.} and Sanchao Liu and O'Brien, {David F.} and Saavedra, {Steven S}",
year = "2003",
month = "5",
doi = "10.1021/bm0256193",
language = "English (US)",
volume = "4",
pages = "841--849",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Planar supported bilayer polymers formed from bis-diene lipids by Langmuir-Blodgett deposition and UV irradiation

AU - Conboy, John C.

AU - Liu, Sanchao

AU - O'Brien, David F.

AU - Saavedra, Steven S

PY - 2003/5

Y1 - 2003/5

N2 - Substrate-supported lipid bilayers have been prepared from bis-diene functionalized phosphorylcholine (PC) lipids and polymerized by UV irradiation. The overall bilayer structure is largely preserved upon removal from water, although significant loss of material occurs from the upper leaflet of the bilayer, likely due to desorption at the air/water interface. The morphology and surface structure of the bilayer, as observed by AFM, indicate a substantially different arrangement of the lipids in the hydrated and dehydrated states, presumably due to the loss of water from the near surface region. These changes have been correlated with infrared spectral shifts sensitive to the conformation of the hydrocarbon chains. Protein adsorption studies show that rehydrated, polymerized bilayers retain a degree of resistance to BSA adsorption intermediate between model hydrophobic and fluid PC lipid bilayer surfaces. The degree of protein adsorption is correlated with desorption of material from the upper leaflet of the bilayer upon drying, which produces voids at which hydrophobically driven protein adsorption occurs.

AB - Substrate-supported lipid bilayers have been prepared from bis-diene functionalized phosphorylcholine (PC) lipids and polymerized by UV irradiation. The overall bilayer structure is largely preserved upon removal from water, although significant loss of material occurs from the upper leaflet of the bilayer, likely due to desorption at the air/water interface. The morphology and surface structure of the bilayer, as observed by AFM, indicate a substantially different arrangement of the lipids in the hydrated and dehydrated states, presumably due to the loss of water from the near surface region. These changes have been correlated with infrared spectral shifts sensitive to the conformation of the hydrocarbon chains. Protein adsorption studies show that rehydrated, polymerized bilayers retain a degree of resistance to BSA adsorption intermediate between model hydrophobic and fluid PC lipid bilayer surfaces. The degree of protein adsorption is correlated with desorption of material from the upper leaflet of the bilayer upon drying, which produces voids at which hydrophobically driven protein adsorption occurs.

UR - http://www.scopus.com/inward/record.url?scp=0037840311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037840311&partnerID=8YFLogxK

U2 - 10.1021/bm0256193

DO - 10.1021/bm0256193

M3 - Article

C2 - 12741807

AN - SCOPUS:0037840311

VL - 4

SP - 841

EP - 849

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 3

ER -