Plant activating sequences: Positively charged peptides are functional as transcriptional activation domains

Juan J. Estruch, Lyle Crossland, Stephen A Goff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Plant sequences that act as transcriptional activation domains in yeast as well as in plants have been isolated by genetic selection in yeast. The selection was based on the reconstitution of a functional GAL4 transcriptional activator. Since the peptides show no homology with reported activation domains, they represent a new class of activating sequences. The sequence P1, which is 10 amino acids long, is the shortest functional activation domain reported. A cDNA that encodes the P14 class (peptides P14-P18) activating sequence have been cloned. The protein exhibits strong homology (higher than 50% amino acid identity) with the BBC1-related sequences, a highly conserved family of basic proteins containing nuclear localization signals. The P14 and P15 peptides are the most effective plant activating sequences. The P14 and P15 peptides are highly hydrophilic, positively charged and mostly unstructured. These properties are at odds with the ones usually found in known activation domains.

Original languageEnglish (US)
Pages (from-to)3983-3989
Number of pages7
JournalNucleic Acids Research
Volume22
Issue number19
StatePublished - Sep 25 1994
Externally publishedYes

Fingerprint

Peptides
Transcriptional Activation
Activation
Chemical activation
Yeasts
Yeast
Amino acids
Amino Acids
Nuclear Localization Signals
Genetic Selection
Proteins
Homology
Complementary DNA
Protein
Odds
CDNA
cell-binding peptide P-15
Class

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Plant activating sequences : Positively charged peptides are functional as transcriptional activation domains. / Estruch, Juan J.; Crossland, Lyle; Goff, Stephen A.

In: Nucleic Acids Research, Vol. 22, No. 19, 25.09.1994, p. 3983-3989.

Research output: Contribution to journalArticle

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