Plant SMU-1 and SMU-2 homologues regulate Pre-mRNA splicing and multiple aspects of development

Taijoon Chung, Dongfang Wang, Cheol Soo Kim, Ramin Yadegari, Brian A. Larkins

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In eukaryotes, alternative splicing of pre-mRNAs contributes significantly to the proper expression of the genome. However, the functions of many auxiliary spliceosomal proteins are still unknown. Here, we functionally characterized plant homologues of nematode suppressors of mec-8 and unc-52 (smu). We compared transcript profiles of maize (Zea mays) smu2 endosperm with those of wild-type plants and identified pre-mRNA splicing events that depend on the maize SMU2 protein. Consistent with a conserved role of plant SMU-2 homologues, Arabidopsis (Arabidopsis thaliana) smu2 mutants also show altered splicing of similar target pre-mRNAs. The Atsmu2 mutants occasionally show developmental phenotypes, including abnormal cotyledon numbers and higher seed weights. We identified AtSMU1 as one of the SMU2-interacting proteins, and Atsmu1 mutations cause similar developmental phenotypes with higher penetrance than Atsmu2. The AtSMU2 and AtSMU1 proteins are localized to the nucleus and highly prevalent in actively dividing tissues. Taken together, our data indicated that the plant SMU-1 and SMU-2 homologues appear to be involved in splicing of specific pre-mRNAs that affect multiple aspects of development.

Original languageEnglish (US)
Pages (from-to)1498-1512
Number of pages15
JournalPlant Physiology
Volume151
Issue number3
DOIs
StatePublished - Nov 2009

Fingerprint

RNA Precursors
Zea mays
Arabidopsis
Proteins
proteins
Phenotype
phenotype
penetrance
mutants
Endosperm
corn
Penetrance
Cotyledon
alternative splicing
Alternative Splicing
Eukaryota
endosperm
eukaryotic cells
cotyledons
Seeds

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Plant SMU-1 and SMU-2 homologues regulate Pre-mRNA splicing and multiple aspects of development. / Chung, Taijoon; Wang, Dongfang; Kim, Cheol Soo; Yadegari, Ramin; Larkins, Brian A.

In: Plant Physiology, Vol. 151, No. 3, 11.2009, p. 1498-1512.

Research output: Contribution to journalArticle

Chung, Taijoon ; Wang, Dongfang ; Kim, Cheol Soo ; Yadegari, Ramin ; Larkins, Brian A. / Plant SMU-1 and SMU-2 homologues regulate Pre-mRNA splicing and multiple aspects of development. In: Plant Physiology. 2009 ; Vol. 151, No. 3. pp. 1498-1512.
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