Plasma fatty acid pattern including diene-conjugated linoleic acid in ethanol users and patients with ethanol-related liver disease

J. Szebeni, C. Eskelson, R. Sampliner, B. Hartmann, J. Griffin, T. Dormandy, Ronald R Watson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The level of conjugated dienes (CD) in the serum, conjugated linoleic acid isomer in the phospholipid fraction [18:2(9,11)], and the fatty acid profile in both the serum and in the separated lipid classes were analyzed in current and previous alcohol abusers and in patients with alcoholic liver disease. None of the subjects consumed alcohol for at least 2-3 days prior to blood collection for analysis of lipids. There was no significant difference in CD or in the absolute level of 18:2(9,11) among the groups, whereas the 18:2(9,11)/18:2(9,12) molar ratio was significantly elevated in patients with liver disease. Fatty acid analysis of the whole plasma extract showed that the absolute level of arachidonic acid increased from 4.8 ± 3.5 mg/dl (n = 9) in lifelong abstainers to 13.4 ± 4.0 (n = 8) in current ethanol abusers, and its relative level (related to the total fatty acids) from 1.73 ± 1.34 to 4.55 ± 1.01 in the same groups. An increased proportion of linoleic acid in the triglyceride and phospholipid fractions from current abusers and patients with liver disease was also found; the percentage of 18:2 in phospholipids increased from 5.4 ± 5.0 (n = 8) in lifelong abstainers to 14.3 ± 3.9 (n = 8) in current abusers and 12.7 ± 2.2 (n = 10) in patients with liver disease. The percentage of 16.0 was significantly lower in the phospholipids of current abusers as compared to lifelong abstainers. These data confirm earlier findings on increased free radical activity in liver diseases, and a lack of it in chronic alcoholics (unless they consume alcohol 1-2 days before the test). The fatty acid profiles suggest a relative accumulation of long chain fatty acids induced by ethanol, conceivably due to a differential effect of ethanol on the β-oxidation and esterification of fatty acids according to their chain length. The observed changes may be useful in biochemical diagnosis of alcohol use.

Original languageEnglish (US)
Pages (from-to)647-650
Number of pages4
JournalAlcoholism: Clinical and Experimental Research
Volume10
Issue number6
StatePublished - 1986
Externally publishedYes

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Conjugated Linoleic Acids
Liver
Liver Diseases
Ethanol
Fatty Acids
Plasmas
Phospholipids
Alcohols
Lipids
Alcoholic Liver Diseases
Esterification
Linoleic Acid
Alcoholics
Serum
Chain length
Arachidonic Acid
Isomers
Free Radicals
Triglycerides
Blood

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Plasma fatty acid pattern including diene-conjugated linoleic acid in ethanol users and patients with ethanol-related liver disease. / Szebeni, J.; Eskelson, C.; Sampliner, R.; Hartmann, B.; Griffin, J.; Dormandy, T.; Watson, Ronald R.

In: Alcoholism: Clinical and Experimental Research, Vol. 10, No. 6, 1986, p. 647-650.

Research output: Contribution to journalArticle

Szebeni, J. ; Eskelson, C. ; Sampliner, R. ; Hartmann, B. ; Griffin, J. ; Dormandy, T. ; Watson, Ronald R. / Plasma fatty acid pattern including diene-conjugated linoleic acid in ethanol users and patients with ethanol-related liver disease. In: Alcoholism: Clinical and Experimental Research. 1986 ; Vol. 10, No. 6. pp. 647-650.
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