Plasma protein denaturation with graded heat exposure

R. Vazquez, Douglas F Larson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

During cardiopulmonary bypass (CPB), perfusion at tepid temperatures (33-35 C) is recommended to avoid high temperature cerebral hyperthermia during and after the operation. However, the ideal temperature for uncomplicated adult cardiac surgery is an unsettled question. Typically, the heat exchanger maximum temperature is monitored between 40-42 C to prevent denaturation of plasma proteins, but studies have not been performed to make these conclusions. Therefore, our hypothesis was to determine the temperature in which blood plasma protein degradation occurs after 2 hours of heat exposure. As a result, blood plasma proteins were exposed to heat in the 37-50 C range for 2 hours. Plasma protein samples were loaded onto an 8-12% gradient gel for SDS-PAGE and low molecular weight plasma protein degradation was detected with graded heat exposure. Protein degradation was first detected between 43-45 C of heat exposure. This study supports the practice of monitoring the heat exchanger between 40-42 C to prevent denaturation of plasma proteins.

Original languageEnglish (US)
Pages (from-to)557-559
Number of pages3
JournalPerfusion
Volume28
Issue number6
DOIs
StatePublished - Nov 2013

Fingerprint

Protein Denaturation
Denaturation
heat
Blood Proteins
Hot Temperature
Proteins
Plasmas
Temperature
Proteolysis
Degradation
Heat exchangers
SDS
Blood
surgery
Surgery
Cardiopulmonary Bypass
monitoring
Thoracic Surgery
Gels
Molecular weight

Keywords

  • degradation
  • denaturation
  • heat exposure
  • plasma protein
  • SDS-PAGE

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging
  • Advanced and Specialized Nursing
  • Safety Research

Cite this

Plasma protein denaturation with graded heat exposure. / Vazquez, R.; Larson, Douglas F.

In: Perfusion, Vol. 28, No. 6, 11.2013, p. 557-559.

Research output: Contribution to journalArticle

Vazquez, R. ; Larson, Douglas F. / Plasma protein denaturation with graded heat exposure. In: Perfusion. 2013 ; Vol. 28, No. 6. pp. 557-559.
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