Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps

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Abstract

Abstract Antimicrobial resistance is one of the most important problems in public health, veterinary medicine and aquaculture. Importantly, plasmid mediated antibiotic resistance of pathogenic Vibrio parahaemolyticus from shrimp can potentially be transferred through transposition, conjugation and plasmid uptake to different bacterial species in aquaculture systems. In this study, we evaluated the antibiotic resistance pattern in V. parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (AHPND) from penaeid shrimp and identified AHPND strains from Mexico showed a high level of resistance to tetracycline (≥5 μg/mL) and have the tetB gene coding tetracycline resistance. In particular, the tetB gene was carried in a single copy plasmid (named as pTetB-VA1) comprising 5162-bp with 40% G + C content from the strain (13-511/A1). The plasmid pTetB-VA1 consists of 9 ORFs encoding tetracycline resistant and repressor proteins, transcriptional regulatory proteins and transposases and showed a 99% sequence identity to other tet gene plasmids (pIS04_68 and pAQU2).

Original languageEnglish (US)
Article number12
Pages (from-to)17-21
Number of pages5
JournalAquaculture Reports
Volume2
DOIs
StatePublished - Nov 1 2015

Fingerprint

Vibrio parahaemolyticus
plasmid
tetracycline
plasmids
necrosis
shrimp
antibiotic resistance
gene
aquaculture systems
aquaculture system
protein
genes
Penaeidae
transposition (genetics)
regulatory proteins
veterinary medicine
aquaculture
open reading frames
public health
Mexico

Keywords

  • Antibiotic
  • Aquaculture
  • Early mortality syndrome (EMS)
  • Minimum inhibitory concentration (MIC)
  • Susceptibility
  • tetB gene

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology

Cite this

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title = "Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps",
abstract = "Abstract Antimicrobial resistance is one of the most important problems in public health, veterinary medicine and aquaculture. Importantly, plasmid mediated antibiotic resistance of pathogenic Vibrio parahaemolyticus from shrimp can potentially be transferred through transposition, conjugation and plasmid uptake to different bacterial species in aquaculture systems. In this study, we evaluated the antibiotic resistance pattern in V. parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (AHPND) from penaeid shrimp and identified AHPND strains from Mexico showed a high level of resistance to tetracycline (≥5 μg/mL) and have the tetB gene coding tetracycline resistance. In particular, the tetB gene was carried in a single copy plasmid (named as pTetB-VA1) comprising 5162-bp with 40{\%} G + C content from the strain (13-511/A1). The plasmid pTetB-VA1 consists of 9 ORFs encoding tetracycline resistant and repressor proteins, transcriptional regulatory proteins and transposases and showed a 99{\%} sequence identity to other tet gene plasmids (pIS04_68 and pAQU2).",
keywords = "Antibiotic, Aquaculture, Early mortality syndrome (EMS), Minimum inhibitory concentration (MIC), Susceptibility, tetB gene",
author = "Han, {Jee Eun} and Mohney, {Leone L.} and Feng-Jyu Tang-Nelson and Pantoja-Morales, {Carlos R} and Lightner, {Donald V}",
year = "2015",
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doi = "10.1016/j.aqrep.2015.04.003",
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T1 - Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps

AU - Han, Jee Eun

AU - Mohney, Leone L.

AU - Tang-Nelson, Feng-Jyu

AU - Pantoja-Morales, Carlos R

AU - Lightner, Donald V

PY - 2015/11/1

Y1 - 2015/11/1

N2 - Abstract Antimicrobial resistance is one of the most important problems in public health, veterinary medicine and aquaculture. Importantly, plasmid mediated antibiotic resistance of pathogenic Vibrio parahaemolyticus from shrimp can potentially be transferred through transposition, conjugation and plasmid uptake to different bacterial species in aquaculture systems. In this study, we evaluated the antibiotic resistance pattern in V. parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (AHPND) from penaeid shrimp and identified AHPND strains from Mexico showed a high level of resistance to tetracycline (≥5 μg/mL) and have the tetB gene coding tetracycline resistance. In particular, the tetB gene was carried in a single copy plasmid (named as pTetB-VA1) comprising 5162-bp with 40% G + C content from the strain (13-511/A1). The plasmid pTetB-VA1 consists of 9 ORFs encoding tetracycline resistant and repressor proteins, transcriptional regulatory proteins and transposases and showed a 99% sequence identity to other tet gene plasmids (pIS04_68 and pAQU2).

AB - Abstract Antimicrobial resistance is one of the most important problems in public health, veterinary medicine and aquaculture. Importantly, plasmid mediated antibiotic resistance of pathogenic Vibrio parahaemolyticus from shrimp can potentially be transferred through transposition, conjugation and plasmid uptake to different bacterial species in aquaculture systems. In this study, we evaluated the antibiotic resistance pattern in V. parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (AHPND) from penaeid shrimp and identified AHPND strains from Mexico showed a high level of resistance to tetracycline (≥5 μg/mL) and have the tetB gene coding tetracycline resistance. In particular, the tetB gene was carried in a single copy plasmid (named as pTetB-VA1) comprising 5162-bp with 40% G + C content from the strain (13-511/A1). The plasmid pTetB-VA1 consists of 9 ORFs encoding tetracycline resistant and repressor proteins, transcriptional regulatory proteins and transposases and showed a 99% sequence identity to other tet gene plasmids (pIS04_68 and pAQU2).

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KW - Early mortality syndrome (EMS)

KW - Minimum inhibitory concentration (MIC)

KW - Susceptibility

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