Platform to Enable Combined Measurement of Dopamine and Neural Activity

Kate L. Parent, Daniel F. Hill, Lindsey M. Crown, Jean Paul Wiegand, Kathleen F. Gies, Michael A. Miller, Christopher W. Atcherley, Michael L Heien, Stephen Leigh Cowen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Complex behaviors depend on the coordination of the activities of ensembles of neurons and the release of neuromodulators such as dopamine. The mechanisms underlying such coordination are not well-understood due to a lack of instrumentation for combined and real-time monitoring of neuromodulator release and the activities of large ensembles of neurons. Here we describe a measurement platform that allows for the combined monitoring of electrophysiology from a high-density electrode array and dopamine dynamics from a carbon-fiber microelectrode. Integration of these two measurement systems was achieved through modification of the existing instrumentation. A shared grounded reference electrode was used in both systems to minimize electrical interference. Further, an optional solid-state-relay array positioned between the electrophysiological electrode array and amplifiers was added to provide additional electrical isolation. The capacity of the integrated measurement platform, termed DANA (Dopamine And Neural Activity), to measure action potentials (high frequency) and local-field oscillations (low frequency) was characterized in vitro using an artificial cerebral spinal fluid gelatin. In vivo recordings from the DANA platform in anesthetized rats demonstrated the ability of the system for near-simultaneous measurement of dopamine release and activity from multiple neurons both in distant brain regions (striatum and hippocampus) and within the same brain region (striatum). Furthermore, this system was shown to be sufficiently compact to measure activity in freely moving animals through recording of single-neuron activity, high-frequency local-field oscillations, and dopamine release.

Original languageEnglish (US)
Pages (from-to)2790-2799
Number of pages10
JournalAnalytical Chemistry
Volume89
Issue number5
DOIs
StatePublished - Mar 7 2017

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Dopamine
Neurons
Electrodes
Neurotransmitter Agents
Brain
Semiconductor relays
Electrophysiology
Monitoring
Microelectrodes
Gelatin
Rats
Animals
Fluids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Parent, K. L., Hill, D. F., Crown, L. M., Wiegand, J. P., Gies, K. F., Miller, M. A., ... Cowen, S. L. (2017). Platform to Enable Combined Measurement of Dopamine and Neural Activity. Analytical Chemistry, 89(5), 2790-2799. https://doi.org/10.1021/acs.analchem.6b03642

Platform to Enable Combined Measurement of Dopamine and Neural Activity. / Parent, Kate L.; Hill, Daniel F.; Crown, Lindsey M.; Wiegand, Jean Paul; Gies, Kathleen F.; Miller, Michael A.; Atcherley, Christopher W.; Heien, Michael L; Cowen, Stephen Leigh.

In: Analytical Chemistry, Vol. 89, No. 5, 07.03.2017, p. 2790-2799.

Research output: Contribution to journalArticle

Parent, KL, Hill, DF, Crown, LM, Wiegand, JP, Gies, KF, Miller, MA, Atcherley, CW, Heien, ML & Cowen, SL 2017, 'Platform to Enable Combined Measurement of Dopamine and Neural Activity', Analytical Chemistry, vol. 89, no. 5, pp. 2790-2799. https://doi.org/10.1021/acs.analchem.6b03642
Parent KL, Hill DF, Crown LM, Wiegand JP, Gies KF, Miller MA et al. Platform to Enable Combined Measurement of Dopamine and Neural Activity. Analytical Chemistry. 2017 Mar 7;89(5):2790-2799. https://doi.org/10.1021/acs.analchem.6b03642
Parent, Kate L. ; Hill, Daniel F. ; Crown, Lindsey M. ; Wiegand, Jean Paul ; Gies, Kathleen F. ; Miller, Michael A. ; Atcherley, Christopher W. ; Heien, Michael L ; Cowen, Stephen Leigh. / Platform to Enable Combined Measurement of Dopamine and Neural Activity. In: Analytical Chemistry. 2017 ; Vol. 89, No. 5. pp. 2790-2799.
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