Pleiotropic effect due to targeted depletion of secretory rhoptry protein ROP2 in Toxoplasma gondii

Valerian Nakaar, Huân M. Ngô, Emily P. Aaronson, Isabelle Coppens, Timothy T. Stedman, Keith A Joiner

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Long after their discovery, the function and biogenesis of rhoptries remain enigmatic. In Apicomplexan parasites, these organelles discharge and their contents are exocytosed at the time of host cell invasion, and are thus proposed to play an essential role in establishing the parasitophorous vacuole. In Toxoplasma gondii, ROP2 is suspected to serve as the molecular link between host cell mitochondria and parasitophorous vacuole membrane. In this study we addressed the function of ROP2. Targeted depletion of ROP2 using a ribozyme-modified antisense RNA strategy resulted in multiple effects on parasite morphology because of a disruption in the formation of mature rhoptries, and an arrest in cytokinesis. The association of host cell mitochondria with the parasitophorous vacuole membrane was abolished and the ROP2-deficient parasites had a reduced uptake of sterol from the host cell. Furthermore, these parasites invaded human fibroblasts poorly and had markedly attenuated virulence in mice. We conclude that rhoptry discharge, and in particular release of ROP2, are essential for parasite invasion, replication and host cell-parasite interaction.

Original languageEnglish (US)
Pages (from-to)2311-2320
Number of pages10
JournalJournal of Cell Science
Volume116
Issue number11
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

Fingerprint

Parasites
Vacuoles
Mitochondria
Host-Parasite Interactions
Antisense RNA
Catalytic RNA
Cytokinesis
Membranes
Toxoplasma
Sterols
Cell Communication
Organelles
Virulence
Fibroblasts
Toxoplasma gondii ROP 2 protein

Keywords

  • Antisense RNA
  • Pleiotropic
  • Rhoptry
  • Secretion
  • Toxoplasma

ASJC Scopus subject areas

  • Cell Biology

Cite this

Pleiotropic effect due to targeted depletion of secretory rhoptry protein ROP2 in Toxoplasma gondii. / Nakaar, Valerian; Ngô, Huân M.; Aaronson, Emily P.; Coppens, Isabelle; Stedman, Timothy T.; Joiner, Keith A.

In: Journal of Cell Science, Vol. 116, No. 11, 01.06.2003, p. 2311-2320.

Research output: Contribution to journalArticle

Nakaar, Valerian ; Ngô, Huân M. ; Aaronson, Emily P. ; Coppens, Isabelle ; Stedman, Timothy T. ; Joiner, Keith A. / Pleiotropic effect due to targeted depletion of secretory rhoptry protein ROP2 in Toxoplasma gondii. In: Journal of Cell Science. 2003 ; Vol. 116, No. 11. pp. 2311-2320.
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