Polyamine-dependent expression of the matrix metalloproteinase matrilysin in a human colon cancer-derived cell line

U. M. Wallon, L. R. Shassetz, Anne E Cress, G. T. Bowden, E. W. Gerner

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Matrilysin, which is a member of the matrix metalloproteinase family and is implicated in colon cancer invasion, is expressed in human colon adenocarcinoma-derived SW1116 cells. We investigated the effect of α-difluoromethylornithine (DFMO) on matrilysin expression in this cell line because others have shown that DFMO can inhibit invasion and carcinogenesis in epithelial tissues, including the colon, in experimental models. DFMO reduced extracellular levels of matrilysin protein after 4 d of treatment. Intracellular levels of matrilysin protein were minimally affected by DFMO treatment. The decrease in extracellular matrilysin protein levels caused by DFMO was not a consequence of lowered steady-state levels of matrilysin mRNA. After 4 d of exposure, the amount of this transcript was higher in DFMO-treated cells than in untreated cultures, whereas the mRNA stabilities were similar. These data show that polyamine depletion by DFMO can suppress the expression of matrilysin, a gene product thought to be involved in tumor invasion. The decrease in extracellular matrilysin protein caused by DFMO treatment appears to be due to a posttranscriptional mechanism, although transcription of this gene also seems to be affected by polyamines in SW1116 cells.

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalMolecular Carcinogenesis
Volume11
Issue number3
DOIs
StatePublished - 1994

Fingerprint

Matrix Metalloproteinase 7
Eflornithine
Polyamines
Matrix Metalloproteinases
Colonic Neoplasms
Cell Line
Colon
Proteins
RNA Stability
Genes
Carcinogenesis
Adenocarcinoma
Theoretical Models
Epithelium
Messenger RNA

Keywords

  • Colon cancer
  • Gene expression
  • Matrilysin
  • Polyamines

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Polyamine-dependent expression of the matrix metalloproteinase matrilysin in a human colon cancer-derived cell line. / Wallon, U. M.; Shassetz, L. R.; Cress, Anne E; Bowden, G. T.; Gerner, E. W.

In: Molecular Carcinogenesis, Vol. 11, No. 3, 1994, p. 138-144.

Research output: Contribution to journalArticle

Wallon, U. M. ; Shassetz, L. R. ; Cress, Anne E ; Bowden, G. T. ; Gerner, E. W. / Polyamine-dependent expression of the matrix metalloproteinase matrilysin in a human colon cancer-derived cell line. In: Molecular Carcinogenesis. 1994 ; Vol. 11, No. 3. pp. 138-144.
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