Polyamine‐dependent expression of the matrix metalloproteinase matrilysin in a human colon cancer—derived cell line

U. Margaretha Wallon, L. Richard Shassetz, Anne E. Cress, G. Tim Bowden, Eugene W. Gerner

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Matrilysin, which is a member of the matrix metalloproteinase family and is implicated in colon cancer invasion, is expressed in human colon adenocarcinoma—derived SW1116 cells. We investigated the effect of α‐difluoromethylornithine (DFMO) on matrilysin expression in this cell line because others have shown that DFMO can inhibit invasion and carcinogenesis in epithelial tissues, including the colon, in experimental models. DFMO reduced extracellular levels of matrilysin protein after 4 d of treatment. Intracellular levels of matrilysin protein were minimally affected by DFMO treatment. The decrease in extracellular matrilysin protein levels caused by DFMO was not a consequence of lowered steady‐state levels of matrilysin mRNA. After 4 d of exposure, the amount of this transcript was higher in DFMO‐treated cells than in untreated cultures, whereas the mRNA stabilities were similar. These data show that polyamine depletion by DFMO can suppress the expression of matrilysin, a gene product thought to be involved in tumor invasion. The decrease in extracellular matrilysin protein caused by DFMO treatment appears to be due to a posttranscriptional mechanism, although transcription of this gene also seems to be affected by polyamines in SW1116 cells. ©1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalMolecular Carcinogenesis
Volume11
Issue number3
DOIs
StatePublished - Nov 1994

Keywords

  • Polyamines
  • colon cancer
  • gene expression
  • matrilysin

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Fingerprint Dive into the research topics of 'Polyamine‐dependent expression of the matrix metalloproteinase matrilysin in a human colon cancer—derived cell line'. Together they form a unique fingerprint.

  • Cite this