Polymerized planar suspended lipid bilayers for single ion channel recordings

Comparison of several dienoyl lipids

Benjamin A. Heitz, Juhua Xu, Ian W. Jones, John P. Keogh, Troy J. Comi, Henry K. Hall, Craig A Aspinwall, Steven S Saavedra

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The stabilization of suspended planar lipid membranes, or black lipid membranes (BLMs), through polymerization of mono- and bis-functionalized dienoyl lipids was investigated. Electrical properties, including capacitance, conductance, and dielectric breakdown voltage, were determined for BLMs composed of mono-DenPC, bis-DenPC, mono-SorbPC, and bis-SorbPC both prior to and following photopolymerization, with diphytanoyl phosphocholine (DPhPC) serving as a control. Poly(lipid) BLMs exhibited significantly longer lifetimes and increased the stability of air-water transfers. BLM stability followed the order bis-DenPC > mono-DenPC > mono-SorbPC > bis-SorbPC. The conductance of bis-SorbPC BLMs was significantly higher than that of the other lipids, which is attributed to a high density of hydrophilic pores, resulting in relatively unstable membranes. The use of poly(lipid) BLMs as matrices for supporting the activity of an ion channel protein (IC) was explored using α-hemolysin (α-HL), a model IC. Characteristic i-V plots of α-HL were maintained following photopolymerization of bis-DenPC, mono-DenPC, and mono-SorbPC, demonstrating the utility of these materials for preparing more durable BLMs for single-channel recordings of reconstituted ICs.

Original languageEnglish (US)
Pages (from-to)1882-1890
Number of pages9
JournalLangmuir
Volume27
Issue number5
DOIs
StatePublished - Mar 1 2011

Fingerprint

Lipid bilayers
Membrane Lipids
Ion Channels
Lipids
lipids
recording
Ions
membranes
Photopolymerization
Electric breakdown
Proteins
Hemolysin Proteins
Phosphorylcholine
proteins
1,2-bis(10(2',4'-hexadienoyloxy)decanoyl)-sn-glycero-3-phosphocholine
Electric properties
Capacitance
Stabilization
Polymerization
Membranes

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Polymerized planar suspended lipid bilayers for single ion channel recordings : Comparison of several dienoyl lipids. / Heitz, Benjamin A.; Xu, Juhua; Jones, Ian W.; Keogh, John P.; Comi, Troy J.; Hall, Henry K.; Aspinwall, Craig A; Saavedra, Steven S.

In: Langmuir, Vol. 27, No. 5, 01.03.2011, p. 1882-1890.

Research output: Contribution to journalArticle

Heitz, Benjamin A. ; Xu, Juhua ; Jones, Ian W. ; Keogh, John P. ; Comi, Troy J. ; Hall, Henry K. ; Aspinwall, Craig A ; Saavedra, Steven S. / Polymerized planar suspended lipid bilayers for single ion channel recordings : Comparison of several dienoyl lipids. In: Langmuir. 2011 ; Vol. 27, No. 5. pp. 1882-1890.
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