Polymorphisms and tissue expression of the feline leukocyte antigen class i loci FLAI-E, FLAI-H, and FLAI-K

Jennifer C. Holmes, Savannah G. Holmer, Peter Ross, Adam S. Buntzman, Jeffrey A Frelinger, Paul R. Hess

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cytotoxic CD8+ T-cell immunosurveillance for intracellular pathogens, such as viruses, is controlled by classical major histocompatibility complex (MHC) class Ia molecules, and ideally, these antiviral T-cell populations are defined by the specific peptide and restricting MHC allele. Surprisingly, despite the utility of the cat in modeling human viral immunity, little is known about the feline leukocyte antigen class I complex (FLAI). Only a few coding sequences with uncertain locus origin and expression patterns have been reported. Of 19 class I genes, three loci - FLAI-E, FLAI-H, and FLAI-K - are predicted to encode classical molecules, and our objective was to evaluate their status by analyzing polymorphisms and tissue expression. Using locus-specific, PCR-based genotyping, we amplified 33 FLAI-E, FLAI-H, and FLAI-K alleles from 12 cats of various breeds, identifying, for the first time, alleles across three distinct loci in a feline species. Alleles shared the expected polymorphic and invariant sites in the α1/α2 domains, and full-length cDNA clones possessed all characteristic class Ia exons. Alleles could be assigned to a specific locus with reasonable confidence, although there was evidence of potentially confounding interlocus recombination between FLAI-E and FLAI-K. Only FLAI-E, FLAI-H, and FLAI-K origin alleles were amplified from cDNAs of multiple tissue types. We also defined hypervariable regions across these genes, which permitted the assignment of names to both novel and established alleles. As predicted, FLAI-E, FLAI-H, and FLAI-K fulfill the major criteria of class Ia genes. These data represent a necessary prerequisite for studying epitope-specific antiviral CD8+ T-cell responses in cats.

Original languageEnglish (US)
Pages (from-to)675-689
Number of pages15
JournalImmunogenetics
Volume65
Issue number9
DOIs
StatePublished - Sep 2013

Fingerprint

Alleles
Cats
feline leukocyte antigen
Major Histocompatibility Complex
T-Lymphocytes
Antiviral Agents
Complementary DNA
MHC Class I Genes
Immunologic Monitoring
Felidae
Genetic Recombination
Genes
Names
Epitopes
Exons
Immunity
Clone Cells
Viruses
Polymerase Chain Reaction
Peptides

Keywords

  • Cat
  • Classical class I genes
  • Major histocompatibility complex
  • Polymorphism

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Polymorphisms and tissue expression of the feline leukocyte antigen class i loci FLAI-E, FLAI-H, and FLAI-K. / Holmes, Jennifer C.; Holmer, Savannah G.; Ross, Peter; Buntzman, Adam S.; Frelinger, Jeffrey A; Hess, Paul R.

In: Immunogenetics, Vol. 65, No. 9, 09.2013, p. 675-689.

Research output: Contribution to journalArticle

Holmes, Jennifer C. ; Holmer, Savannah G. ; Ross, Peter ; Buntzman, Adam S. ; Frelinger, Jeffrey A ; Hess, Paul R. / Polymorphisms and tissue expression of the feline leukocyte antigen class i loci FLAI-E, FLAI-H, and FLAI-K. In: Immunogenetics. 2013 ; Vol. 65, No. 9. pp. 675-689.
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