Population Genetics Provides Evidence for Recombination in Giardia

Margarethe A. Cooper, Rodney D. Adam, Michael Worobey, Charles R Sterling

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Giardia lamblia (syn. Giardia intestinalis, Giardia duodenalis) is an enteric protozoan parasite with two nuclei, and it might be one of the earliest branching eukaryotes [1]. However, the discovery of at least rudimentary forms of certain features, such as Golgi and mitochondria, has refuted the proposal that its emergence from the eukaryotic lineage predated the development of certain eukaryotic features. The recent recognition of many of the genes known to be required for meiosis in the genome has also cast doubt on the idea that Giardia is primitively asexual, but so far there has been no direct evidence of sexual reproduction in Giardia, and population data have suggested clonal reproduction. We did a multilocus sequence evaluation of the genotype A2 reference strain, JH, and five genotype A2 isolates from a highly endemic area in Peru. Loci from different chromosomes yielded significantly different phylogenetic trees, indicating that they do not share the same evolutionary history; within individual loci, tests for recombination yielded significant statistical support for meiotic recombination. These observations provide genetic data supportive of sexual reproduction in Giardia.

Original languageEnglish (US)
Pages (from-to)1984-1988
Number of pages5
JournalCurrent Biology
Volume17
Issue number22
DOIs
StatePublished - Nov 20 2007

Fingerprint

Giardia
Giardia lamblia
Population Genetics
Genetic Recombination
Reproduction
population genetics
Genes
sexual reproduction
varespladib methyl
Mitochondria
Chromosomes
Genotype
loci
Peru
genotype
Meiosis
Eukaryota
meiosis
Protozoa
eukaryotic cells

Keywords

  • EVO_ECOL

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Population Genetics Provides Evidence for Recombination in Giardia. / Cooper, Margarethe A.; Adam, Rodney D.; Worobey, Michael; Sterling, Charles R.

In: Current Biology, Vol. 17, No. 22, 20.11.2007, p. 1984-1988.

Research output: Contribution to journalArticle

Cooper, Margarethe A. ; Adam, Rodney D. ; Worobey, Michael ; Sterling, Charles R. / Population Genetics Provides Evidence for Recombination in Giardia. In: Current Biology. 2007 ; Vol. 17, No. 22. pp. 1984-1988.
@article{869d9853b7074855b6bc138c3d49abe9,
title = "Population Genetics Provides Evidence for Recombination in Giardia",
abstract = "Giardia lamblia (syn. Giardia intestinalis, Giardia duodenalis) is an enteric protozoan parasite with two nuclei, and it might be one of the earliest branching eukaryotes [1]. However, the discovery of at least rudimentary forms of certain features, such as Golgi and mitochondria, has refuted the proposal that its emergence from the eukaryotic lineage predated the development of certain eukaryotic features. The recent recognition of many of the genes known to be required for meiosis in the genome has also cast doubt on the idea that Giardia is primitively asexual, but so far there has been no direct evidence of sexual reproduction in Giardia, and population data have suggested clonal reproduction. We did a multilocus sequence evaluation of the genotype A2 reference strain, JH, and five genotype A2 isolates from a highly endemic area in Peru. Loci from different chromosomes yielded significantly different phylogenetic trees, indicating that they do not share the same evolutionary history; within individual loci, tests for recombination yielded significant statistical support for meiotic recombination. These observations provide genetic data supportive of sexual reproduction in Giardia.",
keywords = "EVO_ECOL",
author = "Cooper, {Margarethe A.} and Adam, {Rodney D.} and Michael Worobey and Sterling, {Charles R}",
year = "2007",
month = "11",
day = "20",
doi = "10.1016/j.cub.2007.10.020",
language = "English (US)",
volume = "17",
pages = "1984--1988",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "22",

}

TY - JOUR

T1 - Population Genetics Provides Evidence for Recombination in Giardia

AU - Cooper, Margarethe A.

AU - Adam, Rodney D.

AU - Worobey, Michael

AU - Sterling, Charles R

PY - 2007/11/20

Y1 - 2007/11/20

N2 - Giardia lamblia (syn. Giardia intestinalis, Giardia duodenalis) is an enteric protozoan parasite with two nuclei, and it might be one of the earliest branching eukaryotes [1]. However, the discovery of at least rudimentary forms of certain features, such as Golgi and mitochondria, has refuted the proposal that its emergence from the eukaryotic lineage predated the development of certain eukaryotic features. The recent recognition of many of the genes known to be required for meiosis in the genome has also cast doubt on the idea that Giardia is primitively asexual, but so far there has been no direct evidence of sexual reproduction in Giardia, and population data have suggested clonal reproduction. We did a multilocus sequence evaluation of the genotype A2 reference strain, JH, and five genotype A2 isolates from a highly endemic area in Peru. Loci from different chromosomes yielded significantly different phylogenetic trees, indicating that they do not share the same evolutionary history; within individual loci, tests for recombination yielded significant statistical support for meiotic recombination. These observations provide genetic data supportive of sexual reproduction in Giardia.

AB - Giardia lamblia (syn. Giardia intestinalis, Giardia duodenalis) is an enteric protozoan parasite with two nuclei, and it might be one of the earliest branching eukaryotes [1]. However, the discovery of at least rudimentary forms of certain features, such as Golgi and mitochondria, has refuted the proposal that its emergence from the eukaryotic lineage predated the development of certain eukaryotic features. The recent recognition of many of the genes known to be required for meiosis in the genome has also cast doubt on the idea that Giardia is primitively asexual, but so far there has been no direct evidence of sexual reproduction in Giardia, and population data have suggested clonal reproduction. We did a multilocus sequence evaluation of the genotype A2 reference strain, JH, and five genotype A2 isolates from a highly endemic area in Peru. Loci from different chromosomes yielded significantly different phylogenetic trees, indicating that they do not share the same evolutionary history; within individual loci, tests for recombination yielded significant statistical support for meiotic recombination. These observations provide genetic data supportive of sexual reproduction in Giardia.

KW - EVO_ECOL

UR - http://www.scopus.com/inward/record.url?scp=36048972596&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36048972596&partnerID=8YFLogxK

U2 - 10.1016/j.cub.2007.10.020

DO - 10.1016/j.cub.2007.10.020

M3 - Article

VL - 17

SP - 1984

EP - 1988

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 22

ER -