Porcine aortic endothelial cells activate human T cells: Direct presentation of MHC antigens and costimulation by ligands for human CD2 and CD28

Allen G. Murray, Mehran M. Khodedoust, Jordan S. Pober, Alfred L.M. Bothwell

Research output: Contribution to journalArticlepeer-review

243 Scopus citations

Abstract

We examined the human xenoresponse to cultured porcine aortic endothelial cells (PAECs). Human CD8+ T cells proliferate to resting MHC class 1-positive PAECs. CD4+ T cells proliferate after MHC class 11 molecules are induced with swine interferon-γ. These responses are greater than corresponding allogeneic responses to human umbilical vein endothelial cells (HUVECs). Limiting dflution analysis shows a 10-fold higher frequency of xenoreactive than alloreactive anti-endothelial lymphocytes. Species-specific monoclonal antibodies suggest that PAECs directly present swine MHC antigens to human T cells and that human CD4 and CD8 molecules participate M this Interaction. Furthermore, PAECs bind CTLA-4-Ig and costimulate human T cells by both the CD2 and CD28 pathways. In contrast, HUVECs do not bind CTLA-4-Ig and only use the CD2 pathway.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalImmunity
Volume1
Issue number1
DOIs
StatePublished - Apr 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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