Possible relationship between chromosome alterations and in vitro cellular radiosensitivity of human malignant melanoma

Heyoung McBride, Baldassare Stea, Anna Hill, Jeffrey Trent

Research output: Contribution to journalArticle

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Abstract

In the present study, in vitro radiation survival analysis was performed on 8 human malignant melanoma cell lines with defined karyotypes. Exponentially growing cells were irradiated to doses of 0 to 8 Gy and examined for soft agar clonogenicity. Two groups emerged from this analysis: 4 cell lines with a small shoulder (extrapolation number, n < 2) and 4 cell lines with a large shoulder (n > 2). Of possible significance, 4 4 cell lines with extrapolation numbers > 2 demonstrated clonal structural abnormalities of chromosome 7. In contrast, 3 4 cell lines with extrapolation numbers < 2 had no structural abnormalities of chromosome 7. These very preliminary results suggest that structural alterations of chromosome 7 may be associated with melanoma tumors with high extrapolation numbers.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume19
Issue number2
DOIs
StatePublished - Aug 1990

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Keywords

  • Cytogenetics
  • Malignant melanoma
  • Radiosensitivity

ASJC Scopus subject areas

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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