Post-ischemic administration of adenosine attenuates renal ischemia-reperfusion injury in a rabbit model

Sreekumar - Subramanian, Mark W. Bowyer, J. Craig Egan, Thomas J. Knolmayer, H. Thomas Lee

Research output: Contribution to journalArticle

Abstract

Introduction: Renal ischemia-reperfusion injury (IRI) is frequently encountered in trauma, transplant and vascular surgery. Adenosine (ADE) is a vasoactive nucleoside shown to attenuate myocardial and skeletal muscle IRI. We evaluated the ability of adenosine, when given after renal ischemia, to reduce renal ischemia-reperftision injury. Methods: Sixteen New Zealand white rabbits were divided into two groups. All rabbits underwent a laparotomy, with bilateral renal artery cross-clamping for 45 minutes, followed by 72 hours of reperfusion. Group 1 (control, N=10) animals received a 0.9% normal saline infusion for the first 4.5 hours of reperfusion. In addition to saline, Group 2 (ADE, N=6) animals received, at 30 minutes after removal of the renal artery clamps, an infusion of ADE 350 μg/kg/min IV for 10 minutes. Baseline and 72-hour serum creatinine (Cr), and blood urea nitrogen (BUN) levels were measured, and the rabbits were sacrificed after 72 hours of reperfusion. Mann-Whitney rank sum statistical analysis was utilized with α set at p < 0.05. Results: There was no significant difference in baseline BUN or Cr between the two groups. Group 1 animals had a significantly greater increase in both Cr (p < 0.006) and BUN (p < 0.003) compared to Group 2, indicating that renal function was preserved in the animals receiving the adenosine infusion. Group 1 Control N = 10 Group 2 ADE N = 6 P Value Cr change (Mean ± 1 SD) 1.56 ± 2.19 -0.07 ± 0.20 < 0.006 BUN change (Mean ± 1 SD) 34.9 ± 37.5 1.33 ± 2.73 < 0.003 Conclusions: Administration of adenosine 30 minutes into reperfusion attenuates renal ischemia-reperfusion injury in this rabbit model. Further studies are necessary to evaluate the feasibility of using adenosine therapeutically in renal ischemia-reperfusion injury.

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number1 SUPPL.
StatePublished - 1999
Externally publishedYes

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Reperfusion Injury
Adenosine
Blood Urea Nitrogen
Rabbits
Kidney
Reperfusion
Creatinine
Renal Artery
Ischemia
Control Groups
Wounds and Injuries
Nucleosides
Constriction
Laparotomy
Blood Vessels
Skeletal Muscle
Transplants
Serum

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Post-ischemic administration of adenosine attenuates renal ischemia-reperfusion injury in a rabbit model. / Subramanian, Sreekumar -; Bowyer, Mark W.; Egan, J. Craig; Knolmayer, Thomas J.; Lee, H. Thomas.

In: Critical Care Medicine, Vol. 27, No. 1 SUPPL., 1999.

Research output: Contribution to journalArticle

Subramanian, Sreekumar - ; Bowyer, Mark W. ; Egan, J. Craig ; Knolmayer, Thomas J. ; Lee, H. Thomas. / Post-ischemic administration of adenosine attenuates renal ischemia-reperfusion injury in a rabbit model. In: Critical Care Medicine. 1999 ; Vol. 27, No. 1 SUPPL.
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abstract = "Introduction: Renal ischemia-reperfusion injury (IRI) is frequently encountered in trauma, transplant and vascular surgery. Adenosine (ADE) is a vasoactive nucleoside shown to attenuate myocardial and skeletal muscle IRI. We evaluated the ability of adenosine, when given after renal ischemia, to reduce renal ischemia-reperftision injury. Methods: Sixteen New Zealand white rabbits were divided into two groups. All rabbits underwent a laparotomy, with bilateral renal artery cross-clamping for 45 minutes, followed by 72 hours of reperfusion. Group 1 (control, N=10) animals received a 0.9{\%} normal saline infusion for the first 4.5 hours of reperfusion. In addition to saline, Group 2 (ADE, N=6) animals received, at 30 minutes after removal of the renal artery clamps, an infusion of ADE 350 μg/kg/min IV for 10 minutes. Baseline and 72-hour serum creatinine (Cr), and blood urea nitrogen (BUN) levels were measured, and the rabbits were sacrificed after 72 hours of reperfusion. Mann-Whitney rank sum statistical analysis was utilized with α set at p < 0.05. Results: There was no significant difference in baseline BUN or Cr between the two groups. Group 1 animals had a significantly greater increase in both Cr (p < 0.006) and BUN (p < 0.003) compared to Group 2, indicating that renal function was preserved in the animals receiving the adenosine infusion. Group 1 Control N = 10 Group 2 ADE N = 6 P Value Cr change (Mean ± 1 SD) 1.56 ± 2.19 -0.07 ± 0.20 < 0.006 BUN change (Mean ± 1 SD) 34.9 ± 37.5 1.33 ± 2.73 < 0.003 Conclusions: Administration of adenosine 30 minutes into reperfusion attenuates renal ischemia-reperfusion injury in this rabbit model. Further studies are necessary to evaluate the feasibility of using adenosine therapeutically in renal ischemia-reperfusion injury.",
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AU - Bowyer, Mark W.

AU - Egan, J. Craig

AU - Knolmayer, Thomas J.

AU - Lee, H. Thomas

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N2 - Introduction: Renal ischemia-reperfusion injury (IRI) is frequently encountered in trauma, transplant and vascular surgery. Adenosine (ADE) is a vasoactive nucleoside shown to attenuate myocardial and skeletal muscle IRI. We evaluated the ability of adenosine, when given after renal ischemia, to reduce renal ischemia-reperftision injury. Methods: Sixteen New Zealand white rabbits were divided into two groups. All rabbits underwent a laparotomy, with bilateral renal artery cross-clamping for 45 minutes, followed by 72 hours of reperfusion. Group 1 (control, N=10) animals received a 0.9% normal saline infusion for the first 4.5 hours of reperfusion. In addition to saline, Group 2 (ADE, N=6) animals received, at 30 minutes after removal of the renal artery clamps, an infusion of ADE 350 μg/kg/min IV for 10 minutes. Baseline and 72-hour serum creatinine (Cr), and blood urea nitrogen (BUN) levels were measured, and the rabbits were sacrificed after 72 hours of reperfusion. Mann-Whitney rank sum statistical analysis was utilized with α set at p < 0.05. Results: There was no significant difference in baseline BUN or Cr between the two groups. Group 1 animals had a significantly greater increase in both Cr (p < 0.006) and BUN (p < 0.003) compared to Group 2, indicating that renal function was preserved in the animals receiving the adenosine infusion. Group 1 Control N = 10 Group 2 ADE N = 6 P Value Cr change (Mean ± 1 SD) 1.56 ± 2.19 -0.07 ± 0.20 < 0.006 BUN change (Mean ± 1 SD) 34.9 ± 37.5 1.33 ± 2.73 < 0.003 Conclusions: Administration of adenosine 30 minutes into reperfusion attenuates renal ischemia-reperfusion injury in this rabbit model. Further studies are necessary to evaluate the feasibility of using adenosine therapeutically in renal ischemia-reperfusion injury.

AB - Introduction: Renal ischemia-reperfusion injury (IRI) is frequently encountered in trauma, transplant and vascular surgery. Adenosine (ADE) is a vasoactive nucleoside shown to attenuate myocardial and skeletal muscle IRI. We evaluated the ability of adenosine, when given after renal ischemia, to reduce renal ischemia-reperftision injury. Methods: Sixteen New Zealand white rabbits were divided into two groups. All rabbits underwent a laparotomy, with bilateral renal artery cross-clamping for 45 minutes, followed by 72 hours of reperfusion. Group 1 (control, N=10) animals received a 0.9% normal saline infusion for the first 4.5 hours of reperfusion. In addition to saline, Group 2 (ADE, N=6) animals received, at 30 minutes after removal of the renal artery clamps, an infusion of ADE 350 μg/kg/min IV for 10 minutes. Baseline and 72-hour serum creatinine (Cr), and blood urea nitrogen (BUN) levels were measured, and the rabbits were sacrificed after 72 hours of reperfusion. Mann-Whitney rank sum statistical analysis was utilized with α set at p < 0.05. Results: There was no significant difference in baseline BUN or Cr between the two groups. Group 1 animals had a significantly greater increase in both Cr (p < 0.006) and BUN (p < 0.003) compared to Group 2, indicating that renal function was preserved in the animals receiving the adenosine infusion. Group 1 Control N = 10 Group 2 ADE N = 6 P Value Cr change (Mean ± 1 SD) 1.56 ± 2.19 -0.07 ± 0.20 < 0.006 BUN change (Mean ± 1 SD) 34.9 ± 37.5 1.33 ± 2.73 < 0.003 Conclusions: Administration of adenosine 30 minutes into reperfusion attenuates renal ischemia-reperfusion injury in this rabbit model. Further studies are necessary to evaluate the feasibility of using adenosine therapeutically in renal ischemia-reperfusion injury.

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