Potassium cyanide protects escherichia coli from complement killing by the inhibition of c3 convertase activity

Earl F. Bloch, Mahnaz Rahbar, Ahmad Karim Wright, Angela M. Patterson, Rhonda F. Souza, Carl H. Hammer, Thelma A. Gaither, Keith A Joiner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The exact mechanism by which deposited C5b-9 complexes kill Gram-negative bacteria is unclear. It has been proposed that during complement activation the membrane attack complex triggers an energy dependent process in Gram-negative bacteria that mediates destruction of the inner membrane. This observation in part resulted from the survival of Gram-negative bacteria that were incubated with an uncoupler (DNP) or an inhibitor (KCN) of oxidative phosphorylation during complement activation. In a reexamination of this issue we employed potassium cyanide (KCN) to block energy dependent pathways and observed a dose dependent inhibition of C9 uptake on E. coli J5 during serum incubation, suggesting that cyanide was interfering with complement activation. To verify the effect on complement activation we chose specifically to study the effects of KCN on the C3 convertase of the classical pathway. Sensitized sheep

Original languageEnglish (US)
Pages (from-to)127-149
Number of pages23
JournalImmunological Investigations
Volume22
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

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Complement C3-C5 Convertases
Potassium Cyanide
Complement Activation
Gram-Negative Bacteria
Escherichia coli
Complement Membrane Attack Complex
Classical Pathway Complement C3 Convertase
Oxidative Phosphorylation
Cyanides
Sheep
Membranes
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Potassium cyanide protects escherichia coli from complement killing by the inhibition of c3 convertase activity. / Bloch, Earl F.; Rahbar, Mahnaz; Wright, Ahmad Karim; Patterson, Angela M.; Souza, Rhonda F.; Hammer, Carl H.; Gaither, Thelma A.; Joiner, Keith A.

In: Immunological Investigations, Vol. 22, No. 2, 1993, p. 127-149.

Research output: Contribution to journalArticle

Bloch, Earl F. ; Rahbar, Mahnaz ; Wright, Ahmad Karim ; Patterson, Angela M. ; Souza, Rhonda F. ; Hammer, Carl H. ; Gaither, Thelma A. ; Joiner, Keith A. / Potassium cyanide protects escherichia coli from complement killing by the inhibition of c3 convertase activity. In: Immunological Investigations. 1993 ; Vol. 22, No. 2. pp. 127-149.
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