Potential shortfall of pyramided transgenic cotton for insect resistance management

Thierry Brévault, Shannon Heuberger, Min Zhang, Christa Ellers-Kirk, Xinzhi Ni, Luke Masson, Xianchun Li, Bruce E Tabashnik, Yves Carriere

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

To delay evolution of pest resistance to transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt), the "pyramid" strategy uses plants that produce two or more toxins that kill the same pest. In the United States, this strategy has been adopted widely, with two-toxin Bt cotton replacing one-toxin Bt cotton. Although two-toxin plants are likely to be more durable than one-toxin plants, the extent of this advantage depends on several conditions. One key assumption favoring success of two-toxin plants is that they kill insects selected for resistance to one toxin, which is called "redundant killing." Here we tested this assumption for a major pest, Helicoverpa zea, on transgenic cotton producing Bt toxins Cry1Ac and Cry2Ab. Selection with Cry1Ac increased survival on two-toxin cotton, which contradicts the assumption. The concentration of Cry1Ac and Cry2Ab declined during the growing season, which would tend toexacerbate this problem. Furthermore, analysis of results from 21 selection experiments with eight species of lepi-dopteran pests indicates that some cross-resistance typically occurs between Cry1A and Cry2A toxins. Incorporation of empirical data into simulation models shows that the observed deviations from ideal conditions could greatly reduce the benefits of the pyramid strategy for pests like H. zea, which have inherently low susceptibility to Bt toxins and have been exposed extensively to one of the toxins in the pyramid before two-toxin plants are adopted. For such pests, the pyramid strategy could be improved by incorporating empirical data on deviations from ideal assumptions about redundant killing and cross-resistance.

Original languageEnglish (US)
Pages (from-to)5806-5811
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number15
DOIs
StatePublished - Apr 9 2013

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Bacillus thuringiensis
Insects
Zea mays
Proteins

Keywords

  • Genetically modified
  • Sustainability

ASJC Scopus subject areas

  • General

Cite this

Potential shortfall of pyramided transgenic cotton for insect resistance management. / Brévault, Thierry; Heuberger, Shannon; Zhang, Min; Ellers-Kirk, Christa; Ni, Xinzhi; Masson, Luke; Li, Xianchun; Tabashnik, Bruce E; Carriere, Yves.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 15, 09.04.2013, p. 5806-5811.

Research output: Contribution to journalArticle

Brévault, Thierry ; Heuberger, Shannon ; Zhang, Min ; Ellers-Kirk, Christa ; Ni, Xinzhi ; Masson, Luke ; Li, Xianchun ; Tabashnik, Bruce E ; Carriere, Yves. / Potential shortfall of pyramided transgenic cotton for insect resistance management. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 15. pp. 5806-5811.
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