Practical considerations for analyzing antigene RNAs (agRNAs): RNA immunoprecipitation of argonaute protein

Jacob C Schwartz, David R. Corey

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Target validation for small RNAs in cells can be a confusing task wrought with pitfalls and false leads. One technique for validating in vivo targets of small RNAs is immunoprecipitation of target RNAs using antibodies again the RNAi machinery. Antigene RNAs (agRNAs) regulate transcription in human cells using machinery from the RNAi regulatory pathway-namely argonaute proteins. Here we describe a technique for validating targets of agRNAs using RNA immunoprecipitation with antibodies against human argonaute proteins. This technique can be used to detect interactions of argonaute proteins in the cell nucleus with their targets, lowly expressed noncoding RNA transcripts.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages301-315
Number of pages15
Volume764
DOIs
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume764
ISSN (Print)10643745

Fingerprint

Argonaute Proteins
Immunoprecipitation
RNA
RNA Interference
Untranslated RNA
Antibodies
Cell Nucleus

Keywords

  • antigene RNAs
  • argonaute
  • Noncoding RNA
  • RNA immunoprecipitation
  • transcriptional gene silencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Schwartz, J. C., & Corey, D. R. (2011). Practical considerations for analyzing antigene RNAs (agRNAs): RNA immunoprecipitation of argonaute protein. In Methods in Molecular Biology (Vol. 764, pp. 301-315). (Methods in Molecular Biology; Vol. 764). https://doi.org/10.1007/978-1-61779-188-8_20

Practical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein. / Schwartz, Jacob C; Corey, David R.

Methods in Molecular Biology. Vol. 764 2011. p. 301-315 (Methods in Molecular Biology; Vol. 764).

Research output: Chapter in Book/Report/Conference proceedingChapter

Schwartz, JC & Corey, DR 2011, Practical considerations for analyzing antigene RNAs (agRNAs): RNA immunoprecipitation of argonaute protein. in Methods in Molecular Biology. vol. 764, Methods in Molecular Biology, vol. 764, pp. 301-315. https://doi.org/10.1007/978-1-61779-188-8_20
Schwartz, Jacob C ; Corey, David R. / Practical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein. Methods in Molecular Biology. Vol. 764 2011. pp. 301-315 (Methods in Molecular Biology).
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