Pre-column derivatization and high-performance liquid chromatography of biogenic amines in blood of normal and malignant hyperthermic pigs

Thomas P. Davis, Charles W. Gehrke, Charles H. Williams, Charles W. Gehrke, Klaus O. Gerhardt

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A sensitive, selective, pre-column derivatization method was used with high-performance liquid chromatography to measure norepinephrine, dopamine and serotonin in plasma from normal and malignant hyperthermic (MH) pigs. Samples were carefully collected from control and stressed animals under halothane anesthesia. Using a simple extraction method involving pre-column derivatization with o-phthalaldehyde and ethyl acetate partitioning, the samples were chromatographed in less than 50 min. Norepinephrine was found to be elevated in MH pigs as the syndrome progressed, reaching levels eight-fold greater than control pigs under anesthesia. These experiments provide some evidence for our hypothesis that a failure to metabolize excess norepinephrine may be one of the key metabolic defects in causing the pathophysiology of the malignant hyperthermia-stress syndrome. The application of our chromatographic method in animal and human tests may provide a pattern of biogenic amine types and levels that could be diagnostic in identifying susceptible humans and carrier animals.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume228
Issue numberC
DOIs
StatePublished - Mar 12 1982

ASJC Scopus subject areas

  • Chemistry(all)

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