Pre-conditioning of smooth muscle cells via induction of the heat shock response limits proliferation following mechanical injury

Marvin J Slepian, Stephen P. Massia, Luke Whitesell

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Arterial smooth muscle cell (SMC) proliferation is a significant component of post-angioplasty restenosis. We evaluated whether pre-conditioning of SMCs, via induction of the heat shock respone prior to actual physical injury, would result in an alteration in cell proliferation following injury. Rat aortic SMCs were pretreated with either chemical or thermal heat shock inducers and then subjected to scrape-wound injury in vitro. Cell proliferation at 24 hrs was measured via 3H-thymidine (Tdr) incorporation and compared with scrape wounded unstressed controls. A significant decline in cell proliferation post scrape-wound injury was observed for both chemical and thermal heat shock pre-conditioned cultures, compared to untreated controls. Increased expression of heat shock protein 72 was confirmed serially throughout the 24 hr study period for both chemical and thermal inducers. Despite reduced proliferation heat shocked cells remained viable as evidenced by fluorescent cell viability assay and preserved migration. Pre-conditioning of SMCs through induction of the heat shock response prior to physical injury may be a useful approach to limit aggressive proliferation observed with mechanical revascularization injury.

Original languageEnglish (US)
Pages (from-to)600-607
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume225
Issue number2
DOIs
StatePublished - Aug 14 1996

Fingerprint

Heat-Shock Response
Smooth Muscle Myocytes
Muscle
Hot Temperature
Cells
Wounds and Injuries
Cell proliferation
Cell Proliferation
Shock
HSP72 Heat-Shock Proteins
Angioplasty
Thymidine
Cell Survival
Rats
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Pre-conditioning of smooth muscle cells via induction of the heat shock response limits proliferation following mechanical injury. / Slepian, Marvin J; Massia, Stephen P.; Whitesell, Luke.

In: Biochemical and Biophysical Research Communications, Vol. 225, No. 2, 14.08.1996, p. 600-607.

Research output: Contribution to journalArticle

@article{bc74e025b4ef4f058faf31a9397a23f1,
title = "Pre-conditioning of smooth muscle cells via induction of the heat shock response limits proliferation following mechanical injury",
abstract = "Arterial smooth muscle cell (SMC) proliferation is a significant component of post-angioplasty restenosis. We evaluated whether pre-conditioning of SMCs, via induction of the heat shock respone prior to actual physical injury, would result in an alteration in cell proliferation following injury. Rat aortic SMCs were pretreated with either chemical or thermal heat shock inducers and then subjected to scrape-wound injury in vitro. Cell proliferation at 24 hrs was measured via 3H-thymidine (Tdr) incorporation and compared with scrape wounded unstressed controls. A significant decline in cell proliferation post scrape-wound injury was observed for both chemical and thermal heat shock pre-conditioned cultures, compared to untreated controls. Increased expression of heat shock protein 72 was confirmed serially throughout the 24 hr study period for both chemical and thermal inducers. Despite reduced proliferation heat shocked cells remained viable as evidenced by fluorescent cell viability assay and preserved migration. Pre-conditioning of SMCs through induction of the heat shock response prior to physical injury may be a useful approach to limit aggressive proliferation observed with mechanical revascularization injury.",
author = "Slepian, {Marvin J} and Massia, {Stephen P.} and Luke Whitesell",
year = "1996",
month = "8",
day = "14",
doi = "10.1006/bbrc.1996.1217",
language = "English (US)",
volume = "225",
pages = "600--607",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Pre-conditioning of smooth muscle cells via induction of the heat shock response limits proliferation following mechanical injury

AU - Slepian, Marvin J

AU - Massia, Stephen P.

AU - Whitesell, Luke

PY - 1996/8/14

Y1 - 1996/8/14

N2 - Arterial smooth muscle cell (SMC) proliferation is a significant component of post-angioplasty restenosis. We evaluated whether pre-conditioning of SMCs, via induction of the heat shock respone prior to actual physical injury, would result in an alteration in cell proliferation following injury. Rat aortic SMCs were pretreated with either chemical or thermal heat shock inducers and then subjected to scrape-wound injury in vitro. Cell proliferation at 24 hrs was measured via 3H-thymidine (Tdr) incorporation and compared with scrape wounded unstressed controls. A significant decline in cell proliferation post scrape-wound injury was observed for both chemical and thermal heat shock pre-conditioned cultures, compared to untreated controls. Increased expression of heat shock protein 72 was confirmed serially throughout the 24 hr study period for both chemical and thermal inducers. Despite reduced proliferation heat shocked cells remained viable as evidenced by fluorescent cell viability assay and preserved migration. Pre-conditioning of SMCs through induction of the heat shock response prior to physical injury may be a useful approach to limit aggressive proliferation observed with mechanical revascularization injury.

AB - Arterial smooth muscle cell (SMC) proliferation is a significant component of post-angioplasty restenosis. We evaluated whether pre-conditioning of SMCs, via induction of the heat shock respone prior to actual physical injury, would result in an alteration in cell proliferation following injury. Rat aortic SMCs were pretreated with either chemical or thermal heat shock inducers and then subjected to scrape-wound injury in vitro. Cell proliferation at 24 hrs was measured via 3H-thymidine (Tdr) incorporation and compared with scrape wounded unstressed controls. A significant decline in cell proliferation post scrape-wound injury was observed for both chemical and thermal heat shock pre-conditioned cultures, compared to untreated controls. Increased expression of heat shock protein 72 was confirmed serially throughout the 24 hr study period for both chemical and thermal inducers. Despite reduced proliferation heat shocked cells remained viable as evidenced by fluorescent cell viability assay and preserved migration. Pre-conditioning of SMCs through induction of the heat shock response prior to physical injury may be a useful approach to limit aggressive proliferation observed with mechanical revascularization injury.

UR - http://www.scopus.com/inward/record.url?scp=0030583239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030583239&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.1217

DO - 10.1006/bbrc.1996.1217

M3 - Article

VL - 225

SP - 600

EP - 607

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -