Precision-cut hamster liver slices as an ex vivo model to study amoebic liver abscess

Pilar Carranza-Rosales, María Guadalupe Santiago-Mauricio, Nancy Elena Guzmán-Delgado, Javier Vargas-Villarreal, Gerardo Lozano-Garza, Javier Ventura-Juárez, Isaías Balderas-Rentería, Javier Morán-Martínez, A Jay Gandolfi

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Entamoeba histolytica is the etiological agent of amoebiasis, the second cause of global morbidity and mortality due to parasitic diseases in humans. In approximately 1% of the cases, amoebas penetrate the intestinal mucosa and spread to other organs, producing extra-intestinal lesions, among which amoebic liver abscess (ALA) is the most common. To study ALA, in vivo and in vitro models are used. However, animal models may pose ethical issues, and are time-consuming and costly; and cell cultures represent isolated cellular lineages. The present study reports the infection of precision-cut hamster liver slices with Entamoeba histolytica trophozoites. The infection time-course, including tissue damage, parallels findings previously reported in the animal model. At the same time amoebic virulence factors were detected in the infected slices. This new model to study ALA is simple and reproducible, and employs less than 1/3 of the hamsters required for in vivo analyses.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalExperimental Parasitology
Volume126
Issue number2
DOIs
Publication statusPublished - Oct 2010

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Keywords

  • Amoebic liver abscess
  • Entamoeba histolytica
  • Ex vivo model
  • Hamster
  • Liver slices
  • Protozoa

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Carranza-Rosales, P., Santiago-Mauricio, M. G., Guzmán-Delgado, N. E., Vargas-Villarreal, J., Lozano-Garza, G., Ventura-Juárez, J., ... Gandolfi, A. J. (2010). Precision-cut hamster liver slices as an ex vivo model to study amoebic liver abscess. Experimental Parasitology, 126(2), 117-125. https://doi.org/10.1016/j.exppara.2010.04.005