Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies

Klaus Brendel, Robyn L. Fisher, Carlos L. Krumdieck, A Jay Gandolfi

Research output: Contribution to journalArticle

Abstract

We describe a novel in vitro mammalian liver system, which simplifies both metabolism and hepatotoxicity studies in a multitude of species. This system is based on mechanical cutting of slices with high precision and culture of such slices under dynamic organ culture conditions. This system permits study of intoxication or metabolism for many hours to days and is suitable for integrative biochemical assays as well as histological and morphological evaluation. We report the toxicity exhibited by the isomeric dichlorobenzenes, which in Sprague-Dawley rat liver slices is M>O>P. We also compared a series of bromobenzene analogs, which in Sprague-Dawley rat liver slices are toxic in the following sequence DBB > BB > BBN > BA > BBT > BT. The toxicity of medium chain length branched-chain fatty acids related to valproic acid also was evaluated in this system. Results show that toxicity increased with chain length in α-methyl fatty acids and depended on the location of the carboxyl group in branched-chain octanoic acids. These examples serve to illustrate the utility of precision-cut rat liver slices in dynamic organ culture for structure–toxicity studies.

Original languageEnglish (US)
Pages (from-to)621-627
Number of pages7
JournalInternational Journal of Toxicology
Volume9
Issue number6
DOIs
StatePublished - 1990

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Organ Culture Techniques
Liver
Toxicity
Rats
Chain length
Metabolism
Sprague Dawley Rats
Fatty Acids
Caprylates
Poisons
Valproic Acid
Assays

ASJC Scopus subject areas

  • Toxicology

Cite this

Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies. / Brendel, Klaus; Fisher, Robyn L.; Krumdieck, Carlos L.; Gandolfi, A Jay.

In: International Journal of Toxicology, Vol. 9, No. 6, 1990, p. 621-627.

Research output: Contribution to journalArticle

Brendel, Klaus ; Fisher, Robyn L. ; Krumdieck, Carlos L. ; Gandolfi, A Jay. / Precision-Cut Rat Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies. In: International Journal of Toxicology. 1990 ; Vol. 9, No. 6. pp. 621-627.
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