The biotransformation of explosives has been investigated by many researchers. Bioremediation of soil and water contaminated with hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is becoming the method of choice for clean-up of a variety of sites. In this study, we investigated biotransformation of RDX in the presence of barium. Ba is a metal commonly found in combination with RDX at sites requiring remediation. RDX was biotransformed by both a consortium of bacteria and an isolate from the consortium under anoxic conditions using a rich medium. However, Ba inhibited cell growth under both aerobic and anoxic conditions and slowed biotransformation rates by 40%. RDX and Ba inhibited growth of the isolate more than growth of the consortium. An additive inhibition model is proposed that accurately predicts the reduced growth rates observed.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology