Preliminary evaluation of transgenic tomato plants expressing a Yersinia pestis antigen fusion protein F1-V Grown in a Greenhouse

R. Matsuda, Chieri Kubota, L. M. Alvarez, G. A. Cardineau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Transgenic plants expressing high-value pharmaceutical protein have great potential for inexpensive and scalable protein production systems. Greenhouse tomato production is a suitable platform for such high-value protein production, achieving high biomass yield, increased containment and environmental control capacity. Our ultimate goal is to develop a greenhouse-based efficient protein production system. In this study, using transgenic tomato plants expressing a predominant antigen fusion protein F1-V against plague as a model system, we characterized growth and development of the transgenic plants under typical semiarid greenhouse conditions and evaluated fruit and protein productivity. Two transgenic lines, 'T22.11' and 'T3D1.2', carrying the transgene f1-v and two non-transgenic tomato cultivars, 'TA234' (wild type) and 'Durinta' (commercial cultivar as reference), were used as the plant material. All plants were grown hydroponically in a greenhouse equipped with a temperature control system from September 2007 to March 2008. Cumulative fruit yield per plant was significantly higher in 'Durinta' than in 'T22.11', 'T3D1.2' and 'TA234', while total soluble-protein (TSP) concentration of fruits of 'Durinta' was significantly lower than those of the other lines. As a result, estimated cumulative TSP production was the lowest in 'Durinta'. This suggests that a tomato cultivar having high biomass productivity is not necessarily suitable for high-value protein production.

Original languageEnglish (US)
Title of host publicationActa Horticulturae
Pages381-386
Number of pages6
Volume797
StatePublished - 2008
EventInternational Workshop on Greenhouse Environmental Control and Crop Production in Semi-Arid Regions - Tucson, AZ, United States
Duration: Oct 20 2008Oct 24 2008

Publication series

NameActa Horticulturae
Volume797
ISSN (Print)05677572

Other

OtherInternational Workshop on Greenhouse Environmental Control and Crop Production in Semi-Arid Regions
CountryUnited States
CityTucson, AZ
Period10/20/0810/24/08

Fingerprint

Yersinia pestis
genetically modified organisms
tomatoes
antigens
greenhouses
proteins
protein value
transgenic plants
production technology
cultivars
biopharmaceuticals
fruits
plague
biomass
fruit yield
transgenes
growth and development
temperature

Keywords

  • Controlled environment agriculture
  • Molecular farming
  • Plague vaccine
  • Plant-made pharmaceuticals
  • Solarium lycopersicum L.

ASJC Scopus subject areas

  • Horticulture

Cite this

Matsuda, R., Kubota, C., Alvarez, L. M., & Cardineau, G. A. (2008). Preliminary evaluation of transgenic tomato plants expressing a Yersinia pestis antigen fusion protein F1-V Grown in a Greenhouse. In Acta Horticulturae (Vol. 797, pp. 381-386). (Acta Horticulturae; Vol. 797).

Preliminary evaluation of transgenic tomato plants expressing a Yersinia pestis antigen fusion protein F1-V Grown in a Greenhouse. / Matsuda, R.; Kubota, Chieri; Alvarez, L. M.; Cardineau, G. A.

Acta Horticulturae. Vol. 797 2008. p. 381-386 (Acta Horticulturae; Vol. 797).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Matsuda, R, Kubota, C, Alvarez, LM & Cardineau, GA 2008, Preliminary evaluation of transgenic tomato plants expressing a Yersinia pestis antigen fusion protein F1-V Grown in a Greenhouse. in Acta Horticulturae. vol. 797, Acta Horticulturae, vol. 797, pp. 381-386, International Workshop on Greenhouse Environmental Control and Crop Production in Semi-Arid Regions, Tucson, AZ, United States, 10/20/08.
Matsuda R, Kubota C, Alvarez LM, Cardineau GA. Preliminary evaluation of transgenic tomato plants expressing a Yersinia pestis antigen fusion protein F1-V Grown in a Greenhouse. In Acta Horticulturae. Vol. 797. 2008. p. 381-386. (Acta Horticulturae).
Matsuda, R. ; Kubota, Chieri ; Alvarez, L. M. ; Cardineau, G. A. / Preliminary evaluation of transgenic tomato plants expressing a Yersinia pestis antigen fusion protein F1-V Grown in a Greenhouse. Acta Horticulturae. Vol. 797 2008. pp. 381-386 (Acta Horticulturae).
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