Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations

Elisabeth Mansfield, Eric E. Ross, Craig A Aspinwall

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Analysis of protein and peptide mixtures via capillary electrophoresis is hindered by nonspecific adsorption of analytes to the capillary walls, resulting in poor separations and quantitative reproducibility. Phospholipid bilayer (PLB) coatings are very promising for improving protein and peptide separations due to the native resistance to nonspecific protein adsorption offered by PLBs; however, these coatings display limited chemical and temporal stability. Here, we show the preparation and characterization of a highly cross-linked, polymerized phospholipid capillary coating prepared using bis-SorbPC. Poly(bis-SorbPC) PLB coatings are prepared in situ within fully enclosed fused silica capillaries via self-assembly and radical polymerization. Polymerization of the PLB coating stabilizes the membrane against desorption from the surface and migration in an electric field, improves the temporal and chemical stability, and allows for the separation of both cationic and anionic proteins, while preserving the native resistance to nonspecific protein adsorption of natural PLBs.

Original languageEnglish (US)
Pages (from-to)3135-3141
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number8
DOIs
StatePublished - Apr 15 2007

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Phospholipids
Coatings
Proteins
Adsorption
Capillary electrophoresis
Peptides
Chemical stability
Fused silica
Free radical polymerization
Self assembly
Desorption
Polymerization
Electric fields
Membranes
1,2-bis(10(2',4'-hexadienoyloxy)decanoyl)-sn-glycero-3-phosphocholine

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. / Mansfield, Elisabeth; Ross, Eric E.; Aspinwall, Craig A.

In: Analytical Chemistry, Vol. 79, No. 8, 15.04.2007, p. 3135-3141.

Research output: Contribution to journalArticle

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