Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations

Elisabeth Mansfield, Eric E. Ross, Craig A. Aspinwall

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Analysis of protein and peptide mixtures via capillary electrophoresis is hindered by nonspecific adsorption of analytes to the capillary walls, resulting in poor separations and quantitative reproducibility. Phospholipid bilayer (PLB) coatings are very promising for improving protein and peptide separations due to the native resistance to nonspecific protein adsorption offered by PLBs; however, these coatings display limited chemical and temporal stability. Here, we show the preparation and characterization of a highly cross-linked, polymerized phospholipid capillary coating prepared using bis-SorbPC. Poly(bis-SorbPC) PLB coatings are prepared in situ within fully enclosed fused silica capillaries via self-assembly and radical polymerization. Polymerization of the PLB coating stabilizes the membrane against desorption from the surface and migration in an electric field, improves the temporal and chemical stability, and allows for the separation of both cationic and anionic proteins, while preserving the native resistance to nonspecific protein adsorption of natural PLBs.

Original languageEnglish (US)
Pages (from-to)3135-3141
Number of pages7
JournalAnalytical chemistry
Volume79
Issue number8
DOIs
StatePublished - Apr 15 2007

ASJC Scopus subject areas

  • Analytical Chemistry

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