Preparation of positional renal slices for study of cell-specific toxicity

Charles E. Ruegg, A. Jay Gandolfi, Raymond B. Nagle, Carlos L. Krumdieck, Klaus Brendel

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

To reduce structural complexity, rabbit kidneys were sliced perpendicular to their cortical-papillary axis to isolate four distinct cell groupings. This positional orientation allows identification of each renal cell type based on its location within the slice. A mechanical slicer was used to make several precision-cut slices rapidly from an oriented cylindrical core of renal tissue, with minimal tissue trauma. Slices were then submerged under a gently circulating oxygenated media in a fritted glass support system that maintains viability (intracellular K+/DNA ratio) and structural integrity (histology) for at least 30 h. A high dose of mercuric chloride (10-3 M) was used to demonstrate the structural and biochemical changes of intoxicated slices. This method provides a controlled subchronic in vitro system for the study of the individual cell types involved in cell-specific renal toxicities and may also be a useful tool for addressing other pharmacological and physiological research questions.

Original languageEnglish (US)
Pages (from-to)111-123
Number of pages13
JournalJournal of Pharmacological Methods
Volume17
Issue number2
DOIs
StatePublished - Apr 1987

Keywords

  • Mechanical slicer
  • Mercuric chloride
  • Renal slices
  • organ culture

ASJC Scopus subject areas

  • Pharmacology

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