Presence of a plant cell wall is not required for transformation of Nicotiana by Agrobacterium tumefaciens

Ebrahim Firoozabady, David W. Galbraith

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Agrobacterium has been used to transform zero to six-day-old cell wall nonregenerating (CWNR) and cell wall regenerating (CWR) leaf protoplasts of tobacco. Transformed cells were selected by phoytohormone autotrophic growth and were verified by detection of the presence of lysopine dehydrogenase. Transformation frequencies in CWNR protoplasts were at least as high as those in CWR protoplasts, indicating that a plant cell wall is not required for the process of crown gall tumorigenesis. Transformation frequencies were highest in two-day-old protoplasts. This age coincides with the onset of DNA synthesis and the first mitosis within the cell populations. We suggest that the initiation of cell cycle activity may be important for the transformation process.

Original languageEnglish (US)
Pages (from-to)175-188
Number of pages14
JournalPlant Cell, Tissue and Organ Culture
Volume3
Issue number2
DOIs
StatePublished - Jun 1 1984
Externally publishedYes

Keywords

  • Agrobacterium tumefaciens
  • cell cycle
  • cell wall
  • crown gall
  • lysopine dehydrogenase
  • transformation

ASJC Scopus subject areas

  • Horticulture

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