Presynaptic dysfunction in drosophila csp mutants

Joy A. Umbach, Konrad E. Zinsmaier, Kai K. Eberle, Erich Buchner, Seymour Benzer, Cameron B. Gundersen

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Cysteine string proteins are synapse-specific proteins. In Drosophila, csp deletion mutants exhibit temperature-sensitive paralysis and early death. Here, we report that neuromuscular transmission is impaired presynaptically in these csp mutant larvae. At 22°C, evoked transmitter release is depressed relative to wild type and rescued controls, and high frequency stimulation of the nerve leads to sporadic failures. At 30°C, stimulus-evoked responses decline gradually before failing completely. When the temperature is returned to 22°C, evoked responses recover. Spontaneous release events persist at both 22°C and 30°C. Since nerve conduction and postsynaptic sensitivity are unaffected, these data indicate that csp mutations disrupt depolarization-secretion coupling. This disruption explains the cellular basis of the temperature-sensitive paralysis of these organisms.

Original languageEnglish (US)
Pages (from-to)899-907
Number of pages9
JournalNeuron
Volume13
Issue number4
DOIs
StatePublished - Oct 1994
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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