Presynaptic regulation of neurotransmission in Drosophila by the G protein-coupled receptor Methuselah

Wei Song, Ravi Ranjan, Ken Dawson-Scully, Peter Bronk, Leo Marin, Laurent Seroude, Yi Jyun Lin, Zhiping Nie, Harold L. Atwood, Seymour Benzer, Konrad E. Zinsmaier

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Abstract

Regulation of synaptic strength is essential for neuronal information processing, but the molecular mechanisms that control changes in neuroexocytosis are only partially known. Here we show that the putative G protein-coupled receptor Methuselah (Mth) is required in the presynaptic motor neuron to acutely upregulate neurotransmitter exocytosis at larval Drosophila NMJs. Mutations in the mth gene reduce evoked neurotransmitter release by ∼50%, and decrease synaptic area and the density of docked and clustered vesicles. Pre- but not postsynaptic expression of normal Mth restored normal release in mth mutants. Conditional expression of Mth restored normal release and normal vesicle docking and clustering but not the reduced size of synaptic sites, suggesting that Mth acutely adjusts vesicle trafficking to synaptic sites.

Original languageEnglish (US)
Pages (from-to)105-119
Number of pages15
JournalNeuron
Volume36
Issue number1
DOIs
StatePublished - Sep 26 2002

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Song, W., Ranjan, R., Dawson-Scully, K., Bronk, P., Marin, L., Seroude, L., Lin, Y. J., Nie, Z., Atwood, H. L., Benzer, S., & Zinsmaier, K. E. (2002). Presynaptic regulation of neurotransmission in Drosophila by the G protein-coupled receptor Methuselah. Neuron, 36(1), 105-119. https://doi.org/10.1016/S0896-6273(02)00932-7