Prevalence of biofilm-forming bacteria in chronic rhinosinusitis

Anthony A. Prince, Jacob D. Steiger, Ayesha N. Khalid, Laurel Dogrhamji, Christine Reger, Steven Eau Claire, Alexander G. Chiu, David W. Kennedy, James N. Palmer, Noam A. Cohen

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

Background: Recently, biofilms have been implicated in the pathogenesis of recalcitrant chronic rhinosinusitis (CRS). We sought to determine the prevalence of biofilm-forming cultures obtained from patients with CRS and clinical factors that may contribute to biofilm formation. Methods: Endoscopically guided sinonasal cultures were obtained in duplicate from CRS patients with evidence of mucopurulence. Bacterial swabs were sent for microbiological characterization and were simultaneously evaluated for biofilm-forming capacity by a modified Calgary Biofilm Detection Assay. Biofilm formation was based on concomitant values of biofilm-forming Pseudomonas aeruginosa O1 (PAO1) (positive control) and non-biofilm-forming mutants sad-31 (type IV pili) and sad-36 (flagella K; negative control). Samples, with growth greater than the sad-31 mutant, were designated as biofilm formers. Results: Sinonasal cultures were obtained from 157 consecutive patients (83 female patients) over a 4-month period. Forty-five samples (28.6%) showed biofilm formation. Among patients with a prior history of functional endoscopic sinus surgery (FESS), 30.7% (n = 42) showed biofilm growth. For patients naive to surgical intervention (n = 20), only 15% showed biofilm formation. A positive, statistically significant correlation existed between biofilm formation and number of prior FESS procedures. Polymicrobial cultures, Pseudomonas aeruginosa, and/or Staphylococcus aureus comprised 71% of samples. Chi-squared analysis showed an association with prior infections, but not with any pharmacologic therapy or comorbidies. Conclusion: We show a high percentage of CRS patients (28.6%) whose sinonasal mucopurulence has biofilm-forming capacity. Postsurgical patients had a high prevalence of biofilm-forming bacteria, a possible reflection of the severe nature of their disease. Additional studies are warranted.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalAmerican Journal of Rhinology
Volume22
Issue number3
DOIs
StatePublished - May 1 2008
Externally publishedYes

Keywords

  • Biofilms
  • Calgary biofilm detection
  • Chronic rhinosinusitis
  • Functional endoscopic sinus surgery
  • Pseudomonas
  • Staphylococcus

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Prince, A. A., Steiger, J. D., Khalid, A. N., Dogrhamji, L., Reger, C., Claire, S. E., Chiu, A. G., Kennedy, D. W., Palmer, J. N., & Cohen, N. A. (2008). Prevalence of biofilm-forming bacteria in chronic rhinosinusitis. American Journal of Rhinology, 22(3), 239-245. https://doi.org/10.2500/ajr.2008.22.3180