Prevalence of necrotizing hepatopancreatitis in female broodstock of white shrimp Penaeus vannamei with unilateral eyestalk ablation and hormone injection

M. S Morales Covarrubias, A. G. Osuna-Duarte, A. Garcia-Gasca, Donald V Lightner, J. C. Mota-Urbina

Research output: Contribution to journalArticle

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Abstract

The present study analyzed the relationship between unilateral eyestalk ablation and steroid hormone injection in female white shrimp Penaeus vannamei, the prevalence of necrotizing hepatopancreatitis (NHP) caused by the intracellular rickettsia-like bacterium NHP-B. Two simultaneous bioassays determined the onset and development of NHP in broodstock. Wet-mount analysis, conventional histopathology, and in situ hybridization with an NHP-B-specific DNA probe were used to assess the prevalence and degree of severity of NHP and to confirm NHP-B infection. Wet-mount and histopathological results showed that females with unilateral eyestalk ablation had a 64% prevalence of NHP at grades 2 and 4, while females receiving the hormone injection showed a 23% prevalence of NHP at grades 3 and 4; control shrimp injected with phosphate-buffered saline (PBS) displayed a 9% prevalence of NHP at grade 3, whereas nonablated controls showed no signs of disease. The rickettsia-like specific DNA probe demonstrated a positive hybridization signal in NHP-B, and the hepatopancreas, the target tissue for the bacterium, showed a strong positive signal. Significant differences (P < 0.05) were found in females with unilateral eyestalk ablation compared with nonablated controls, in hormone-injected females compared with PBS-injected controls, and in females with unilateral eyestalk ablation compared with the hormone injection group. We conclude that unilateral eyestalk ablation enhances NHP disease prevalence in broodstock reared under maturation conditions.

Original languageEnglish (US)
Pages (from-to)19-25
Number of pages7
JournalJournal of Aquatic Animal Health
Volume18
Issue number1
DOIs
StatePublished - Mar 2006

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necrotizing hepatopancreatitis
Penaeidae
breeding stock
Litopenaeus vannamei
ablation
hormone
shrimp
hormones
Hormones
injection
Injections
Rickettsia
DNA Probes
Phosphates
probe
phosphate
Hepatopancreas
disease prevalence
Bacteria
DNA

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

Prevalence of necrotizing hepatopancreatitis in female broodstock of white shrimp Penaeus vannamei with unilateral eyestalk ablation and hormone injection. / Covarrubias, M. S Morales; Osuna-Duarte, A. G.; Garcia-Gasca, A.; Lightner, Donald V; Mota-Urbina, J. C.

In: Journal of Aquatic Animal Health, Vol. 18, No. 1, 03.2006, p. 19-25.

Research output: Contribution to journalArticle

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abstract = "The present study analyzed the relationship between unilateral eyestalk ablation and steroid hormone injection in female white shrimp Penaeus vannamei, the prevalence of necrotizing hepatopancreatitis (NHP) caused by the intracellular rickettsia-like bacterium NHP-B. Two simultaneous bioassays determined the onset and development of NHP in broodstock. Wet-mount analysis, conventional histopathology, and in situ hybridization with an NHP-B-specific DNA probe were used to assess the prevalence and degree of severity of NHP and to confirm NHP-B infection. Wet-mount and histopathological results showed that females with unilateral eyestalk ablation had a 64{\%} prevalence of NHP at grades 2 and 4, while females receiving the hormone injection showed a 23{\%} prevalence of NHP at grades 3 and 4; control shrimp injected with phosphate-buffered saline (PBS) displayed a 9{\%} prevalence of NHP at grade 3, whereas nonablated controls showed no signs of disease. The rickettsia-like specific DNA probe demonstrated a positive hybridization signal in NHP-B, and the hepatopancreas, the target tissue for the bacterium, showed a strong positive signal. Significant differences (P < 0.05) were found in females with unilateral eyestalk ablation compared with nonablated controls, in hormone-injected females compared with PBS-injected controls, and in females with unilateral eyestalk ablation compared with the hormone injection group. We conclude that unilateral eyestalk ablation enhances NHP disease prevalence in broodstock reared under maturation conditions.",
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